A sequential separation strategy for facile isolation and comprehensive analysis of human urine N-glycoproteome

Analytical and Bioanalytical Chemistry
Bianbian HuoXiaohong Qian

Abstract

Urine is an attractive and non-invasive alternative source to tissue, blood or other biofluids for biomarker screening in clinical research. In normal human adult urine, 48% of the total urinary protein is in the sediment, 49% is soluble and the remaining 3% is contained in urinary extracellular vesicles (EVs). The soluble proteins and EV proteins in urine have attracted particular attention in recent years as cancer diagnostics. Furthermore, considering the important role of N-glycoproteins in practically all physiological processes, including regulating receptor-ligand binding, cell-cell interactions, inflammatory response and tumour progression, N-glycoproteome in human urine is an invaluable target for monitoring the physiological status and pathological changes of the kidney and urinary tract. Given the different origins of the soluble proteins and EV proteins in the urine, different N-glycoproteome patterns exist. Therefore, isolating the soluble N-glycoproteins and EV N-glycoproteins for separate analysis will provide a more specific and comprehensive view and provide a deeper understanding of human urinary N-glycoproteome. In this work, we developed a sequential separation method that isolates urinary soluble proteins a...Continue Reading

References

Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Trairak PisitkunMark A Knepper
Dec 1, 2005·Molecular & Cellular Proteomics : MCP·Linjie WangYouhe Gao
Jul 14, 2006·Molecular & Cellular Proteomics : MCP·Trairak PisitkunMark A Knepper
Jan 19, 2007·American Journal of Physiology. Renal Physiology·Anita CheruvankyRobert A Star
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
May 6, 2011·Mass Spectrometry Reviews·Pyong-Gon MoonMoon-Chang Baek
Feb 19, 2014·Nature Reviews. Rheumatology·Edit I BuzasSteffen Gay
Apr 4, 2014·American Journal of Physiology. Renal Physiology·Mahdi SalihEwout J Hoorn
Aug 5, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Carlamaria ZojaGiuseppe Remuzzi
Dec 3, 2014·Molecular & Cellular Proteomics : MCP·Mayank SaraswatRisto Renkonen
Jan 27, 2015·Annual Review of Pathology·Sean R StowellRichard D Cummings
May 30, 2015·Nature Cell Biology·Yun Zhang, Xiao-Fan Wang
Aug 21, 2015·Nature Reviews. Cancer·Salomé S Pinho, Celso A Reis
Nov 3, 2015·Nature Neuroscience·Kirti SharmaMikael Simons
Nov 5, 2016·Trends in Endocrinology and Metabolism : TEM·Isabel Huang-DoranAntonio Vidal-Puig
Sep 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Mengxi WuTony Jun Huang
Sep 25, 2017·Methods in Enzymology·Qiang Yang, Lai-Xi Wang
Oct 31, 2017·Nature Reviews. Nephrology·Michael L MerchantJon B Klein
Nov 15, 2017·Nature Communications·Sophia DollMatthias Mann
Jan 13, 2018·Arthritis Research & Therapy·Chi Chiu MokChandra Mohan

❮ Previous
Next ❯

Citations

Mar 17, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Piyawan PaisrisarnYoshinobu Baba
Jun 4, 2020·Cancers·Manuel Castanheira de OliveiraRicardo Ribeiro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nan fang yi ke da xue xue bao = Journal of Southern Medical University
Tai-Xue An, Lei Zheng
Nefrología : publicación oficial de la Sociedad Española Nefrologia
Jordi RoviraMaría José Ramírez-Bajo
International Journal of Molecular Sciences
Chiara CiardielloDolores Di Vizio
© 2022 Meta ULC. All rights reserved