A sequestration feedback determines dynamics and temperature entrainment of the KaiABC circadian clock.

Molecular Systems Biology
Christian BrettschneiderMarkus Kollmann

Abstract

The circadian rhythm of the cyanobacterium Synechococcus elongatus is controlled by three proteins, KaiA, KaiB, and KaiC. In a test tube, these proteins form complexes of various stoichiometry and the average phosphorylation level of KaiC exhibits robust circadian oscillations in the presence of ATP. Using mathematical modeling, we were able to reproduce quantitatively the experimentally observed phosphorylation dynamics of the KaiABC clockwork in vitro. We thereby identified a highly non-linear feedback loop through KaiA inactivation as the key synchronization mechanism of KaiC phosphorylation. By using the novel method of native mass spectrometry, we confirm the theoretically predicted complex formation dynamics and show that inactivation of KaiA is a consequence of sequestration by KaiC hexamers and KaiBC complexes. To test further the predictive power of the mathematical model, we reproduced the observed phase synchronization dynamics on entrainment by temperature cycles. Our model gives strong evidence that the underlying entrainment mechanism arises from a temperature-dependent change in the abundance of KaiAC and KaiBC complexes.

References

Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·T NishiwakiT Kondo
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hideo IwasakiTakao Kondo
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya MoriCarl H Johnson
Mar 10, 2004·The Journal of Biological Chemistry·Sheng YeJames C Sacchettini
Jun 1, 2004·Nature Structural & Molecular Biology·Tatsuya UzumakiMasahiro Ishiura
Jul 2, 2004·Nature·Irina MihalcescuStanislas Leibler
Sep 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yao XuCarl Hirschie Johnson
Sep 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Taeko NishiwakiTakao Kondo
Feb 21, 2006·Physical Review Letters·Eldon Emberly, Ned S Wingreen
Sep 15, 1957·Proceedings of the National Academy of Sciences of the United States of America·J W Hastings, B M Sweeney
Jul 26, 2006·PLoS Computational Biology·Arun MehraPeter Ruoff
Nov 1, 2006·Analytical Chemistry·Robert H H van den HeuvelAlbert J R Heck
Mar 14, 2007·Molecular Systems Biology·Sébastien ClodongMarkus Kollmann
Mar 29, 2007·PLoS Biology·Tetsuya MoriCarl Hirschie Johnson
Apr 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jeroen S van ZonPieter Rein ten Wolde
May 4, 2007·PloS One·Mitsumasa YodaMasaki Sasai
Oct 30, 2007·Nature Structural & Molecular Biology·Hiroshi ItoTakao Kondo
May 15, 2008·Genes & Development·Yohko KitayamaTakao Kondo
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rebecca J RoseSheena E Radford
Oct 31, 2008·Nature Reviews. Molecular Cell Biology·Béla Novák, John J Tyson
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Takuya YoshidaTakao Kondo

❮ Previous
Next ❯

Citations

Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yong-Gang ChangAndy LiWang
May 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tetsuhiro S Hatakeyama, Kunihiko Kaneko
Mar 22, 2013·PLoS Computational Biology·Stefanie HertelIlka M Axmann
Jun 7, 2014·Annual Review of Biochemistry·Carl Hirschie Johnson, Martin Egli
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Joost SnijderAlbert J R Heck
Feb 22, 2011·Annual Review of Biophysics·Carl Hirschie JohnsonMartin Egli
Mar 21, 2013·Molecular Systems Biology·Peter D GouldAnthony J W Hall
Apr 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Joris PaijmansDavid K Lubensky
Jun 17, 2015·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Martyn AmosFriedrich Simmel
Feb 8, 2013·Cell Cycle·Rekha PattanayekMartin Egli
Jan 7, 2014·Marine Genomics·Ilka M AxmannAnnegret Wilde
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jenny LinMichael J Rust
Mar 17, 2012·Developmental Cell·Steven A BrownRobert Dallmann
Mar 16, 2017·PLoS Computational Biology·Joris PaijmansPieter Rein Ten Wolde
Jan 14, 2017·Frontiers in Bioengineering and Biotechnology·Stefanie Westermark, Ralf Steuer
Nov 18, 2018·Interface Focus·Nicolas M Schmelling, Ilka M Axmann
Mar 28, 2012·Journal of Experimental Botany·Ralf SteuerRainer Machné
Mar 24, 2017·Journal of the Royal Society, Interface·Martin Egli
Nov 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Lu HongAaron R Dinner
Jun 18, 2014·The Journal of Biological Chemistry·Martin Egli
May 13, 2014·Acta Crystallographica. Section D, Biological Crystallography·Rekha PattanayekMartin Egli
Aug 1, 2020·Frontiers in Immunology·Aïssatou Bailo DialloJean-Louis Mege
Jul 13, 2017·Biophysical Journal·Joris PaijmansPieter Rein Ten Wolde

❮ Previous
Next ❯

Software Mentioned

matlab

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.