A series of surveys on assay for anti-A/B antibody by Japanese ABO-incompatible Transplantation Committee

Xenotransplantation
Takaaki Kobayashi, Kazuhide Saito

Abstract

The measurement of anti-blood groups A and B antibody (Ab) titers is considered to be important in ABO-incompatible transplantation. However, no standard method for quantitative determination has yet been established in Japan. Inter-examiner variability was likely because Ab titer was determined mainly by visual observation. In order to assess inter-institutional variation in the measurement of anti-A/B Ab titer, a series of surveys was conducted by the Japanese ABO-incompatible Transplantation Committee. In 2003, the first national survey was conducted. Serum samples from six healthy volunteers with blood groups A (n = 2), B (n = 2) and O (n = 2) were sent to 29 institutions and anti-A/B Ab titer was measured by their customary methods. Isohemagglutinin assay in serial-doubling dilutions of serum using a test tube was widely used in all institutions. Inter-institutional difference between maximum and minimum value reached as much as 32-fold in immunoglobulin M (IgM) and 256-fold in IgG. As detailed protocol for assay seemed to be different between institutions, we attempted to standardize the protocol based on the result of a questionnaire survey. In 2004, a second survey was conducted in the same manner as the previous one, e...Continue Reading

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