A serine protease isolated from the bristles of the Amazonic caterpillar, Premolis semirufa, is a potent complement system activator

PloS One
Isadora Maria Villas BoasDenise V Tambourgi

Abstract

The caterpillar of the moth Premolis semirufa, commonly named pararama, is found in the Brazilian Amazon region. Accidental contact with the caterpillar bristles causes an intense itching sensation, followed by symptoms of an acute inflammation, which last for three to seven days after the first incident. After multiple accidents a chronic inflammatory reaction, called "Pararamose", characterized by articular synovial membrane thickening with joint deformities common to chronic synovitis, frequently occurs. Although complement mediated inflammation may aid the host defense, inappropriate or excessive activation of the complement system and generation of anaphylatoxins can lead to inflammatory disorder and pathologies. The aim of the present study was to evaluate, in vitro, whether the Premolis semirufa's bristles extract could interfere with the human complement system. The bristles extract was able to inhibit the haemolytic activity of the alternative pathway, as well as the activation of the lectin pathway, but had no effect on the classical pathway, and this inhibition seemed to be caused by activation and consumption of complement components. The extract induced the production of significant amounts of all three anaphylatox...Continue Reading

References

Jan 1, 1986·Complement·T E Hugli
Jul 1, 1985·The Journal of Investigative Dermatology·H J Müller-Eberhard
Jan 1, 1980·Toxicon : Official Journal of the International Society on Toxinology·G EggertsenJ Sjöquist
Jul 1, 1995·Revista Da Sociedade Brasileira De Medicina Tropical·J J da SilvaA V de Oliveira
Jul 1, 1994·British Journal of Pharmacology·D V TambourgiT L Kipnis
May 1, 1994·Analytical Biochemistry·D E Kleiner, W G Stetler-Stevenson
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D MastellosJ D Lambris
Apr 5, 2001·The New England Journal of Medicine·M J Walport
Sep 17, 2003·The Journal of Experimental Medicine·Christoph W StreyJohn D Lambris
Sep 30, 2004·Nature Immunology·Michael C Carroll
Apr 15, 2005·International Immunopharmacology·Daniela Trinca BertazziEliane Candiani Arantes
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erin Addis-LieserMónica G Chiaramonte
May 30, 2009·Molecular Immunology·Andreas KlosJörg Köhl
Jul 16, 2009·Inflammation & Allergy Drug Targets·Qi PengWuding Zhou
Apr 27, 2010·Toxicon : Official Journal of the International Society on Toxinology·Carl-Wilhelm Vogel, David C Fritzinger
Aug 3, 2010·Molecular Immunology·Giselle Pidde-QueirozDenise V Tambourgi
Jan 18, 2012·BMC Immunology·Gabriela D TanakaDenise V Tambourgi
Apr 17, 2013·The Journal of Biological Chemistry·Mariko Honda-OgawaShigetada Kawabata
Jul 11, 2013·Journal of Innate Immunity·Monika JuskoAnna M Blom
Jul 8, 2014·Molecular Immunology·Denise V Tambourgi, Carmen W van den Berg

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Citations

Jun 4, 2020·Biomedicines·Andrea SeldeslachtsJan Tytgat
Aug 18, 2018·Journal of Immunology Research·Marie DelafontaineDenise V Tambourgi

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Methods Mentioned

BETA
Protein Assay
ELISA
Fluorescence
gel filtration
electrophoresis
zymography

Software Mentioned

GraphPad Prism

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