A serological aptamer-assisted proximity ligation assay for COVID-19 diagnosis and seeking neutralizing aptamers.

Chemical Science
Ran LiuJingjing Zhang

Abstract

Rapid and accurate diagnosis of COVID-19 plays an essential role in the current epidemic prevention and control. Despite the promise of nucleic acid and antibody tests, there is still a great challenge to reduce the misdiagnosis, especially for asymptomatic individuals. Here we report a generalizable method for highly specific and ultrasensitive detection of serum COVID-19-associated antigens based on an aptamer-assisted proximity ligation assay. The sensor is based on binding two aptamer probes to the same protein target that brings the ligation DNA region into close proximity, thereby initiating ligation-dependent qPCR amplification. Using this system, serum nucleocapsid protein has been detected quantitatively by converting protein recognition into a detectable qPCR signal using a simple, homogeneous and fast detection workflow in ∼2 hours. In addition, this system has also been transformed into a universal platform for measuring specific interactions between spike S1 and its receptor ACE2, and more importantly demonstrated the feasibility for screening and investigation of potential neutralizing aptamers. Since in vitro selection can obtain aptamers selective for many COVID-19-associated antigens, the method demonstrated he...Continue Reading

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Citations

May 22, 2021·ACS Sensors·Hao ChenJaebum Choo
Jun 3, 2021·Nanomaterials·Jafar AliMohamed M Mohamed
Jun 5, 2021·Advanced Healthcare Materials·Luyao ShenYonggang Ke
Apr 1, 2021·Emergent Materials·E Alperay TarimH Cumhur Tekin
Apr 15, 2021·Chemistry, an Asian Journal·Cong HanLiyun Zhang
Aug 12, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mina ZareSeeram Ramakrishna
Oct 19, 2021·ACS Applied Materials & Interfaces·Zhong Wei JiangCheng Zhi Huang
Nov 10, 2021·Analytical Methods : Advancing Methods and Applications·Mukti MandalGorachand Dutta

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
proximity ligation
proximity ligation assay
PCR
electrophoresis
proximity

Software Mentioned

Apt

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