A serological assay to detect SARS-CoV-2 seroconversion in humans.

Nature Medicine
Fatima AmanatFlorian Krammer

Abstract

Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.

References

Mar 5, 2016·Nature·Robert N KirchdoerferAndrew B Ward
Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jesper PallesenJason S McLellan
Jan 30, 2020·The New England Journal of Medicine·Qun LiZijian Feng
Feb 23, 2020·Science·Daniel WrappJason S McLellan
Mar 11, 2020·Cell·Alexandra C WallsDavid Veesler
Mar 27, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Anu HaveriCarita Savolainen-Kopra
Mar 29, 2020·JAMA : the Journal of the American Medical Association·Chenguang ShenLei Liu
Apr 9, 2020·Emerging Infectious Diseases·Nisreen M A OkbaBart L Haagmans
Apr 18, 2020·Current Protocols in Microbiology·Daniel StadlbauerFlorian Krammer
Jun 9, 2020·BioRxiv : the Preprint Server for Biology·Saahir KhanUNKNOWN Prometheus Study Group

❮ Previous
Next ❯

Citations

May 18, 2020·Science·Florian Krammer, Viviana Simon
May 10, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Tulika SinghSallie R Permar
Jun 17, 2020·Emerging Infectious Diseases·Hannah ClaphamClarence C Tam
Jun 26, 2020·Current Protocols in Microbiology·Fatima AmanatFlorian Krammer
Jul 10, 2020·Journal of Histotechnology·Seth Malloy, Yongfu Wang
Aug 5, 2020·The Journal of Experimental Medicine·Benjamin IsraelowAkiko Iwasaki
Jun 20, 2020·Nature·Davide F RobbianiMichel C Nussenzweig
Jul 7, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·William B StubblefieldWesley H Self
Jun 28, 2020·Journal of Virology·John P Moore, P J Klasse
Aug 9, 2020·The Journal of Infectious Diseases·Gerco den HartogRobert S van Binnendijk
Aug 9, 2020·Allergy·Anurag BhallaParameswaran Nair
Sep 5, 2020·Advanced Healthcare Materials·Eduardo Ruiz-HitzkyPilar Aranda
Jul 15, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kara L LynchAlan H B Wu
May 24, 2020·The FEBS Journal·Cassandra L AtzrodtThomas P Clements
Sep 25, 2020·Current Opinion in HIV and AIDS·Ying-Ying SuNing-Shao Xia
Sep 24, 2020·Nature·Florian Krammer
Jun 5, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Xiaoli WangQuanyi Wang
Jun 26, 2020·American Journal of Clinical Pathology·Andrea P EspejoCarolyn Cray
Jul 17, 2020·Science Immunology·Leticia Kuri-CervantesMichael R Betts
Aug 6, 2020·Sensors·Heba A HusseinFerdinando Febbraio
Aug 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·T Alex PerkinsMarya Poterek
Sep 19, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jan C HolterTom E Mollnes
Aug 21, 2020·American Journal of Clinical Pathology·Clarence W ChanKiang-Teck J Yeo
Sep 5, 2020·Biological Procedures Online·Elena EkramiMatin Mamoudifard
Sep 5, 2020·BMJ : British Medical Journal·Stephen BurgessDipender Gill
Sep 26, 2020·Frontiers in Medicine·Ariel D StockEmad Eskandar
Sep 9, 2020·Clinical and Translational Medicine·Meng XuXiaobo Yu
Sep 29, 2020·Frontiers in Medicine·Ina P PavlovaAsh K Tewari
Aug 15, 2020·Clinical Microbiology Reviews·Daniele FocosiMarco Tuccori
Oct 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·Angela M Bosco-LauthRichard A Bowen
Aug 15, 2020·Nature Communications·Antonio E MuruatoPei-Yong Shi

❮ Previous
Next ❯

Datasets Mentioned

BETA
MN908947.3
MT020880

Methods Mentioned

BETA
enzyme-linked immunosorbent assays
electrophoresis
ELISA
ELISAs
transfections

Clinical Trials Mentioned

NCT04264858

Software Mentioned

GraphPad Prism
iSpike
mSpike

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

MedRxiv : the Preprint Server for Health Sciences
Fatima AmanatThomas Moran
MedRxiv : the Preprint Server for Health Sciences
Fatima AmanatFlorian Krammer
© 2021 Meta ULC. All rights reserved