A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots

Scientific Reports
Donna Grace KarpCheng-Ting Tsai

Abstract

Accurate surveillance of coronavirus disease 2019 (COVID-19) incidence requires large-scale testing of the population. Current testing methods require in-person collection of biospecimens by a healthcare worker, limiting access of individuals who do not have access to testing facilities while placing both patients and healthcare workers at risk of exposure to infection. We report the development and validation of a at-home finger-prick dried blood spot collection kit and an analysis method. We demonstrated 100% sensitivity and specificity using at-home collected specimens across the US. Such methods may facilitate the conduct of unbiased serosurveys within hard to reach populations and help reduce the sample collection burden of serological testing on both health care systems and individuals alike.

References

Apr 12, 2016·ACS Central Science·Cheng-Ting TsaiCarolyn R Bertozzi
Dec 19, 2017·The Journal of Allergy and Clinical Immunology·Cheng-Ting TsaiStephen J Galli
Jan 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Ting TsaiCarolyn R Bertozzi
Jan 30, 2020·The New England Journal of Medicine·Qun LiZijian Feng
Feb 20, 2020·The New England Journal of Medicine·Marc LipsitchLyn Finelli
Feb 20, 2020·The New England Journal of Medicine·Lirong ZouJie Wu
Apr 5, 2020·Journal of Clinical Microbiology·Yi-Wei TangCharles W Stratton
Apr 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Kai DuanXiaoming Yang
Apr 9, 2020·Emerging Infectious Diseases·Nisreen M A OkbaBart L Haagmans
May 1, 2020·Nature Medicine·Quan-Xin LongAi-Long Huang
May 14, 2020·Nature Medicine·Fatima AmanatFlorian Krammer
Jun 9, 2020·MedRxiv : the Preprint Server for Health Sciences·Jeffrey D WhitmanAlexander Marson
Jun 9, 2020·MedRxiv : the Preprint Server for Health Sciences·Michael JoynerArturo Casadevall

❮ Previous
Next ❯

Citations

Feb 16, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ranya MulchandaniUNKNOWN EDSAB-HOME Study Investigators
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Panagiotis-Dimitrios MingasTomaž Vovk
Jun 12, 2021·Diagnostic Microbiology and Infectious Disease·Coleman T TurgeonElitza S Theel
Aug 3, 2021·Journal of Pharmaceutical and Biomedical Analysis·Olivier SalaminTiia Kuuranne
Aug 20, 2021·Laboratory Medicine·Elizabeth Smerczak
Aug 17, 2021·Human Antibodies·David BakerAngray S Kang
Oct 21, 2021·Microbiology Spectrum·Hannah L ItellJulie Overbaugh

❮ Previous
Next ❯

Methods Mentioned

BETA
dried blood spot collection
pull down
PCR

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The American Journal of Nursing
Torey Sippio-James
Current Opinion in Pediatrics
Scott M WeissmanErica Ramos
Current Opinion in Pediatrics
Scott M WeissmanErica Ramos
© 2021 Meta ULC. All rights reserved