PMID: 7537702Mar 15, 1995Paper

A serotype-specific epitope of dengue virus 1 identified by phage displayed random peptide library

FEMS Microbiology Letters
Z J YaoM C Chung

Abstract

From a panel of monoclonal antibodies of dengue viruses, a serotype-specific epitope of dengue virus 1 was screened from a random peptide library displayed on phage. The epitope was the determinant reactive with monoclonal antibody 15F3-1 that was specific to dengue 1. The screening was monitored by a dot blotting procedure, and after three rounds of screening a consensus motif, HRYSWK, was found. This sequence matches the sequence HKYSWK, corresponding to the amino acid residues 885-890 of polyprotein or residues 111-116 of the non-structural protein 1 of dengue virus serotype 1. The linear epitope was confirmed by testing the antigenicity of chemically synthesized 8-branched peptide.

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Citations

Dec 1, 1995·Immunotechnology : an International Journal of Immunological Engineering·D H Du PlessisF Jordaan
Mar 26, 2004·Virus Research·Paola R Barrero, Alicia S Mistchenko
Sep 19, 1998·Clinical and Diagnostic Virology·M A BrintonF A Ennis
Jul 17, 1998·Journal of Neurology, Neurosurgery, and Psychiatry·K H RandK M Heilman
Apr 2, 2005·Protein Expression and Purification·Ravulapalli AnandaRaoNavin Khanna
Nov 1, 1996·International Journal of Peptide and Protein Research·Z J YaoM C Chung
Apr 30, 2014·The American Journal of Tropical Medicine and Hygiene·Magot Diata OmokokoTakeshi Kurosu
Jul 3, 2021·Pharmaceuticals·Bryan GibbChristine L Schneider

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