A SERS Optophysiological Probe for the Real-Time Mapping and Simultaneous Determination of the Carbonate Concentration and pH Value in a Live Mouse Brain

Angewandte Chemie
Weikang WangYang Tian

Abstract

To have a profound understanding of the physiological and pathological processes in a brain, both chemical and electrical signals need to be recorded, but this is still very challenging. Herein, micrometer- to nanometer-sized SERS optophysiological probes were created to determine both the CO32- concentration and the pH in live brains and neurons because both species play important roles in regulating the acid-base balance in the brain. A ratiometric SERS microarray of eight microprobes with tip sizes of 5 μm was established and used for the first time for real-time mapping and simultaneous quantification of CO32- and pH in a live brain. We found that both the CO32- concentration and the pH value dramatically decreased under ischemic conditions. The present SERS technique can be combined with electrophysiology without cross-talk to record both electrical and chemical signals in brains. To deepen our understanding of the mechanism of ischemia on the single-cell level, a SERS nanoprobe with a tip size of 200 nm was developed for use in a single neuron.

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Citations

Oct 3, 2020·The Analyst·Gregory Q Wallace, Jean-François Masson
Oct 3, 2020·The Journal of Chemical Physics·Gregory Q WallaceJean-François Masson
May 25, 2021·Frontiers in Chemistry·Aleksandra Szaniawska, Andrzej Kudelski
Dec 23, 2020·ACS Applied Materials & Interfaces·Xiaojia LiuXing Ma
Jun 24, 2020·Journal of the American Chemical Society·Aimin GeAndrew A Peterson
Nov 26, 2020·ACS Applied Materials & Interfaces·Xingjuan ZhaoJean-Francois Masson
Feb 20, 2020·ACS Sensors·Xiaotong GuoLingxin Chen
Sep 11, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaojia LiuXing Ma

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