A SERS/fluorescence dual-mode nanosensor based on the human telomeric G-quadruplex DNA: Application to mercury (II) detection

Biosensors & Bioelectronics
Min LiuYiman Liu

Abstract

DNA-metal nanoparticle conjugates have been increasingly exploited for sensing purposes over the past decades. However, most of the existing strategies are operated with canonical DNA structures, such as single-stranded forms, stem-loop structures, and double helix structures. There is intense interest in the development of nano-system based on high order DNA secondary structures. Herein, we propose a SERS/fluorescence dual-mode nanosensor, where the signal transduction mechanism is based on the conformational switching of the human telomeric G-quadruplex DNA. The nanosensor exhibits excellent SERS/fluorescence responses to the complementary strands of G-quadruplexes. Based on T-Hg(2+)-T coordination chemistry, this sensor is effectively applied to determination of Hg(2+) in buffer solution and real samples. It achieves a limit of detection (LOD) as low as 1ppt, which is ~100 times more sensitive than conventional optical sensors. We anticipate that the proposed G-quadruplex-based nanosensor could be applied to the analysis of other metal ions and small molecules in environmental samples and biological systems.

References

Oct 20, 1989·Science·A R Rahmouni, R D Wells
Jan 24, 2003·Nature·Helen Pearson
Mar 12, 2008·Biochimie·Aklank JainKaren M Vasquez

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Citations

Feb 24, 2016·Journal of Environmental Sciences (China)·Zhenli SunChuanyong Jing
Sep 12, 2015·Biosensors & Bioelectronics·Lanying LiGang Liu
Apr 4, 2018·Small·Yiman LiuMin Liu
Nov 6, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Bin ZhouQing-Li Suo
Jun 14, 2021·Biosensors & Bioelectronics·Yaofeng ZhouYonghua Xiong
Nov 19, 2015·ACS Applied Materials & Interfaces·Min LiuYiman Liu

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