A serum-free medium formulation efficiently supports isolation and propagation of canine adipose-derived mesenchymal stem/stromal cells

PloS One
Laxminarayana R DevireddyLynne Boxer

Abstract

Medium containing Fetal Bovine Serum (FBS) provides a supportive environment for isolation and expansion of mesenchymal stromal/stem cells (MSCs); however, the inherent variability of FBS may contribute to inconsistencies in cell growth and yield between batches of stem cell products. For this reason, we set out to develop a serum-free medium capable of supporting the in vitro expansion of MSCs. First a naïve serum-free medium was formulated by Sato's approach. Once it was established that the naïve serum-free medium supported the expansion of canine adipose-derived MSCs (Ad-MSCs), the serum-free medium was optimized by addition of growth factors. Combinations of growth factors were chosen and compared by their effect on cell proliferation and colony formation. Growth characteristics of canine adipose-derived MSCs cultured in the serum-free medium were comparable to those cultured in standard FBS containing medium. In addition, cell surface marker expression and differentiation potential of serum-free and FBS-based cultures were also comparable. However, a commercial serum-free medium developed for human MSC culture did not support growth of canine Ad-MSCs. In summary, canine Ad-MSCs isolated and cultured in serum-free medium r...Continue Reading

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Citations

Sep 14, 2018·Current Stem Cell Research & Therapy·Ali GolchinAbdolreza Ardeshirylajimi
Nov 2, 2019·International Journal of Molecular Sciences·Heba AbdelrazikAlessandra Pelagalli
Apr 2, 2021·Frontiers in Bioengineering and Biotechnology·Weifeng LiTian-Yun Wang
May 7, 2021·Stem Cells International·Dina RadyKarim M Fawzy El-Sayed
Jun 23, 2021·Tissue Engineering. Part C, Methods·Zhuoming LiuLaxminarayana R Devireddy
Jul 17, 2021·Tissue Engineering. Part a·Nolutho MkhumbeniKeolebogile Shirley Caroline Motaung
Jan 19, 2022·Langmuir : the ACS Journal of Surfaces and Colloids·Fatih Inci

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Methods Mentioned

BETA
flow cytometry
light microscopy

Software Mentioned

GraphPad

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