PMID: 2501784Jul 1, 1989Paper

A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions

Proceedings of the National Academy of Sciences of the United States of America
C G Cupples, J H Miller

Abstract

We describe the construction of six strains of Escherichia coli with different mutations at the same coding position in the lacZ gene, which specifies the active site glutamic acid residue at position 461 in beta'-galactosidase. Each strain is Lac- and reverts to Lac+ only by restoring the glutamic acid codon. The strains have been designed so that each reverts via one of the six base substitutions. The set of strains allows detection of each transition and transversion simply by monitoring the Lac- to Lac+ frequency, as demonstrated here with characterized mutagens and mutator alleles. These strains are useful for rapidly determining the mutagenic specificity of mutagens at a single site, for detecting low levels of stimulation of certain base substitutions, for monitoring specific base changes in response to various experimental conditions or strain backgrounds, and for isolating new mutator strains.

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Dec 15, 1977·Journal of Molecular Biology·C Coulondre, J H Miller
Jan 1, 1976·Annual Review of Biochemistry·J W Drake, R H Baltz
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y NghiemJ H Miller
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R M Schaaper, R L Dunn
Mar 5, 1985·Journal of Molecular Biology·J H Miller
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B N AmesF D Lee
Sep 5, 1973·Journal of Molecular Biology·L Prakash, F Sherman
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·C YanofskyV Horn
Feb 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·C YanofskyV Horn
Jan 1, 1983·Annual Review of Genetics·J H Miller
Nov 25, 1981·Journal of Molecular Biology·J H MillerM Hofer
Jun 1, 1961·Journal of Molecular Biology·F JACOB, J MONOD

❮ Previous
Next ❯

Citations

Dec 1, 1994·Mutation Research·K NegishiH Hayatsu
Jan 7, 2004·DNA Repair·Grace LeeJeffrey H Miller
Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Mar 26, 2005·Mutation Research·Anetta NowosielskaElzbieta Grzesiuk
Dec 19, 2002·Journal of Molecular Biology·Peter FriedhoffAlfred Pingoud
Dec 13, 2000·Mutation Research·P D Josephy
Oct 23, 1997·Mutation Research·W JiM Ehrlich
Nov 7, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L D ClaxtonK T Chung
Nov 26, 2002·FEMS Microbiology Letters·Antonio OliverJesús Blázquez
Nov 7, 1996·Gene·J H Miller, M Michaels
Jun 1, 1997·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·M Bouzon, P Marlière
Mar 1, 1997·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·M Bouzon, P Marlière
Jun 27, 2001·Mutation Research·S BjellandE Seeberg
Jul 25, 1998·Mutation Research·B A Bridges
Jul 31, 2003·Mutation Research·Igor B Rogozin, Youri I Pavlov
Apr 26, 2003·DNA Repair·Susan S WallaceJeffrey P Bond
Jan 6, 2010·Journal of the American Chemical Society·Joseph C GenereuxJacqueline K Barton
Apr 23, 2002·Molecular Microbiology·Robert DoraziM Zafri Humayun
Nov 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·D ParkhomchukV Ogryzko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.