A seven-gene expression panel distinguishing clonal expansions of pre-leukemic and chronic lymphocytic leukemia B cells from normal B lymphocytes

Immunologic Research
Brian A McCarthyNicholas Chiorazzi

Abstract

Chronic lymphocytic leukemia (CLL) is a clonal disease of B lymphocytes manifesting as an absolute lymphocytosis in the blood. However, not all lymphocytoses are leukemic. In addition, first-degree relatives of CLL patients have an ~15 % chance of developing a precursor condition to CLL termed monoclonal B cell lymphocytosis (MBL), and distinguishing CLL and MBL B lymphocytes from normal B cell expansions can be a challenge. Therefore, we selected FMOD, CKAP4, PIK3C2B, LEF1, PFTK1, BCL-2, and GPM6a from a set of genes significantly differentially expressed in microarray analyses that compared CLL cells with normal B lymphocytes and used these to determine whether we could discriminate CLL and MBL cells from B cells of healthy controls. Analysis with receiver operating characteristics and Bayesian relevance determination demonstrated good concordance with all panel genes. Using a random forest classifier, the seven-gene panel reliably distinguished normal polyclonal B cell populations from expression patterns occurring in pre-CLL and CLL B cell populations with an error rate of 2 %. Using Bayesian learning, the expression levels of only two genes, FMOD and PIK3C2B, correctly distinguished 100 % of CLL and MBL cases from normal p...Continue Reading

References

Mar 1, 1979·Investigative Radiology·J A Swets
Jan 1, 1984·Medical Decision Making : an International Journal of the Society for Medical Decision Making·B J McNeil, J A Hanley
Nov 25, 2003·Journal of Chemical Information and Computer Sciences·Vladimir SvetnikBradley P Feuston
Jun 3, 2004·The Lancet Infectious Diseases·Samuel Yang, Richard E Rothman
Feb 25, 2005·The New England Journal of Medicine·Nicholas ChiorazziManlio Ferrarini
Jun 2, 2005·The Journal of Cell Biology·Tania MaffucciMarco Falasca
Jun 21, 2005·Current Opinion in Pharmacology·Len StephensPhillip Hawkins
Oct 14, 2005·Journal of Cellular Physiology·Matthew Wheeler, Jan Domin
Jan 19, 2006·Clinical Microbiology Reviews·M J EspyT F Smith
Aug 30, 2006·British Journal of Haematology·Robert A Kyle, S Vincent Rajkumar
Jan 4, 2007·The Journal of Clinical Investigation·Victoria Del Gaizo MooreAnthony Letai
Nov 21, 2007·British Journal of Haematology·Gerald MartiRobert F Vogt
Jun 20, 2008·Computer Methods and Programs in Biomedicine·Wen ZhangXihao Hu
Aug 9, 2008·The New England Journal of Medicine·Andy C RawstronPeter Hillmen
Sep 6, 2008·Science·D Williams ParsonsKenneth W Kinzler
Feb 14, 2009·The New England Journal of Medicine·Ola LandgrenNeil E Caporaso
Apr 7, 2009·Leukemia & Lymphoma·Tait Shanafelt, Curtis A Hanson
Dec 4, 2009·Nature Reviews. Cancer·Thorsten ZenzStephan Stilgenbauer
Sep 28, 2010·American Journal of Hematology·Maurizio FrezzatoFrancesco Rodeghiero
Dec 1, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xinjiang CaiEdward Y Skolnik
Apr 27, 2013·International Reviews of Immunology·Tadeusz Robak, Pawel Robak
Sep 17, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fortunato MorabitoAntonino Neri
Apr 25, 2015·American Journal of Hematology·Michael Hallek

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Citations

Aug 28, 2016·Oncotarget·Hilmar QuentmeierHans G Drexler
May 20, 2020·Cell Adhesion & Migration·Joanna PastwińskaJarosław Dastych
May 23, 2018·Nature Medicine·Renée BeekmanJose I Martin-Subero
Jun 15, 2019·Cancer Cell International·Mohammad Hossein PourhanifehHamed Mirzaei
Dec 1, 2020·Expert Review of Hematology·Marc Sorigue

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