A shelf-stable fluorogenic isothermal amplification assay for the detection of Burkholderia pseudomallei

The Analyst
Jilien Michelle Wong Tzeling, Chan Yean Yean

Abstract

A shelf-stable loop-mediated isothermal amplification (LAMP) reagent for Burkholderia pseudomallei detection is described. The coupling of LAMP reagents with the indirect colorimetric indicator and consequently its lyophilization enable the simple evaluation of results without the need for any advance laboratory instruments. The reagents were found to have a stable shelf life of at least 30 days with well-maintained sensitivity and specificity.

References

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