A shell matrix protein of Pinctada mazatlanica produces nacre platelets in vitro

Scientific Reports
Crisalejandra Rivera-PerezNorma Y Hernandez-Saavedra

Abstract

Nacre is the main component of the pearl oyster shells and it is synthesized by specialized soluble and insoluble shell matrix proteins. Insoluble proteins from the decalcification of the shell are the less studied proteins due to the technical problems to isolate them from the organic matrix. In this study, an insoluble shell matrix protein from Pinctada mazatlanica, pearlin (Pmaz-pearlin), was successfully cloned from the mantle tissue, and the native protein isolated from the shell was functionally characterized. The full coding sequence of Pmaz-pearlin mRNA consists of 423 base pairs, which encode to a 16.3 kDa pearlin. Analysis of the deduced amino acid sequence revealed that Pmaz-pearlin contained four acidic regions, an NG repeat domain, and Cys conserved residues, the latter potentially forms four disulfide bridges which might stabilize the protein structure. The isolated protein from the shell is a glycoprotein of ~ 16.74 kDa which can produce aragonite and calcite crystals in vitro. Our results show that Pmaz-pearlin is a well-conserved protein involved in nacre layer growth, which produces calcite crystals in the presence of CaCl2, aragonite crystal polymorphs with a hexagonal structure in the presence of MgCl2, and ...Continue Reading

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Citations

Jun 29, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Crisalejandra Rivera-Pérez, Norma Y Hernández-Saavedra

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Datasets Mentioned

BETA
MT746149
AB020779
AB032612

Methods Mentioned

BETA
electrophoresis
glycosylation
PCR
Scanning Electron Microscopy

Software Mentioned

NetPhos2
MEGA
ORF
CLUSTAL Omega
BLAST
SignalP
DiANNA
ClustalW
NetNGlyc
NetOGly

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