A Shift in Central Metabolism Accompanies Virulence Activation in Pseudomonas aeruginosa.

MBio
Kumar PerinbamAlbert Siryaporn

Abstract

The availability of energy has significant impact on cell physiology. However, the role of cellular metabolism in bacterial pathogenesis is not understood. We investigated the dynamics of central metabolism during virulence induction by surface sensing and quorum sensing in early-stage biofilms of the multidrug-resistant bacterium Pseudomonas aeruginosa We established a metabolic profile for P. aeruginosa using fluorescence lifetime imaging microscopy (FLIM), which reports the activity of NADH in live cells. We identified a critical growth transition period during which virulence is activated. We performed FLIM measurements and direct measurements of NADH and NAD+ concentrations during this period. Here, planktonic (low-virulence) and surface-attached (virulence-activated) populations diverged into distinct metabolic states, with the surface-attached population exhibiting FLIM lifetimes that were associated with lower levels of enzyme-bound NADH and decreasing total NAD(H) production. We inhibited virulence by perturbing central metabolism using citrate and pyruvate, which further decreased the enzyme-bound NADH fraction and total NAD(H) production and suggested the involvement of the glyoxylate pathway in virulence activation ...Continue Reading

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Citations

Nov 21, 2020·Nature Reviews. Microbiology·Elio RossiHelle Krogh Johansen
Feb 13, 2021·Sensors·Jenu V ChackoKevin W Eliceiri
Mar 9, 2021·Frontiers in Molecular Biosciences·Fabrice Jean-PierreGeorge A O'Toole
Jul 13, 2021·Frontiers in Cellular and Infection Microbiology·Morgan A AlfordRobert E W Hancock

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Methods Mentioned

BETA
fluorescence lifetime imaging microscopy
Fluorescence
fluorescence microscopy
scraping

Software Mentioned

Matlab
FLIM
SimFCS

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