A short conserved motif in ALYREF directs cap- and EJC-dependent assembly of export complexes on spliced mRNAs

Nucleic Acids Research
Agnieszka M GromadzkaNiels H Gehring

Abstract

The export of messenger RNAs (mRNAs) is the final of several nuclear posttranscriptional steps of gene expression. The formation of export-competent mRNPs involves the recruitment of export factors that are assumed to facilitate transport of the mature mRNAs. Using in vitro splicing assays, we show that a core set of export factors, including ALYREF, UAP56 and DDX39, readily associate with the spliced RNAs in an EJC (exon junction complex)- and cap-dependent manner. In order to elucidate how ALYREF and other export adaptors mediate mRNA export, we conducted a computational analysis and discovered four short, conserved, linear motifs present in RNA-binding proteins. We show that mutation in one of the new motifs (WxHD) in an unstructured region of ALYREF reduced RNA binding and abolished the interaction with eIF4A3 and CBP80. Additionally, the mutation impaired proper localization to nuclear speckles and export of a spliced reporter mRNA. Our results reveal important details of the orchestrated recruitment of export factors during the formation of export competent mRNPs.

References

Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I Edery, N Sonenberg
Mar 1, 1996·Computers & Chemistry·P BucherK Hofmann
Jan 13, 2001·Genes & Development·D LibriJ Boulay
Mar 22, 2001·The Journal of Biological Chemistry·I C BraunE Izaurralde
Apr 5, 2002·Molecular Cell·Mutsuhito OhnoIain W Mattaj
Jul 9, 2002·Molecular and Cellular Biology·Isabelle C BraunElisa Izaurralde
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Heather L WiegandBryan R Cullen
May 29, 2004·The Journal of Biological Chemistry·Sébastien DegotCatherine Tomasetto
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jul 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peter Tompa, Peter Csermely
Jun 10, 2005·FEBS Letters·Victor Neduva, Robert B Russell
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Jul 7, 2005·Genes & Development·Seiji MasudaRobin Reed
Sep 12, 2006·Current Opinion in Biotechnology·Victor Neduva, Robert B Russell
Dec 28, 2006·Cell·Hong ChengRobin Reed
Mar 17, 2007·The Journal of Biological Chemistry·Takayuki NojimaMasatoshi Hagiwara
Nov 7, 2007·Molecular and Cellular Biology·Ichiro Taniguchi, Mutsuhito Ohno
Dec 12, 2007·Nature Reviews. Genetics·Suzanne Komili, Pamela A Silver
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patricia ValenciaRobin Reed
Mar 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Guillaume M HautbergueStuart A Wilson
Feb 17, 2009·Molecular Cell·Yongsheng ShiJames L Manley
May 5, 2009·Cell·Niels H GehringMatthias W Hentze
Oct 20, 2009·Current Biology : CB·Guillaume M HautbergueStuart A Wilson
Feb 4, 2010·Nucleic Acids Research·Kuo-Ming LeeWoan-Yuh Tarn
Sep 13, 2011·Molecular BioSystems·Norman E DaveyToby J Gibson

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Citations

Dec 23, 2016·Wiley Interdisciplinary Reviews. RNA·Lauren A WoodwardGuramrit Singh
May 24, 2017·Cell Research·Dan Dominissini, Gideon Rechavi
Sep 9, 2018·The Journal of Cell Biology·Ke WangHong Cheng
Jan 21, 2018·Biochemical Society Transactions·Jennifer V Gerbracht, Niels H Gehring
Sep 30, 2016·The Biochemical Journal·Catherine G HeathStuart A Wilson
May 29, 2020·Nucleic Acids Research·Mireille MelkoTorben Heick Jensen
Sep 5, 2020·Essays in Biochemistry·Jianshu Wang, Hong Cheng
Sep 23, 2020·Nucleic Acids Research·Yuhui DouTorben Heick Jensen
Mar 21, 2017·Cellular and Molecular Life Sciences : CMLS·Petra Björk, Lars Wieslander
Nov 13, 2019·Wiley Interdisciplinary Reviews. RNA·William Garland, Torben Heick Jensen
Apr 29, 2018·Nature Communications·Wiebke Manuela SchulzeStephen Cusack
Jan 31, 2019·Scientific Reports·Anita BoisraméCécile Neuvéglise
Jan 9, 2020·The Biochemical Journal·Asaf Ashkenazy-TitelmanRalph H Kehlenbach
Sep 16, 2018·Scientific Reports·Simon LesbirelStuart A Wilson
Sep 22, 2017·Nucleic Acids Research·Min ShiHong Cheng
Sep 17, 2020·Biomolecules·Kathi ZarnackKatja Sträßer
May 15, 2018·Frontiers in Molecular Neuroscience·Chiara PaolantoniCorinna Giorgi
Jun 11, 2020·Biomolecules·Lena P Schlautmann, Niels H Gehring
Jan 10, 2020·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Ken-Ichi FujitaSeiji Masuda
Sep 17, 2021·Nucleic Acids Research·Yimin WangHong Cheng

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