A Short History and Description of Drosophila melanogaster Classical Genetics: Chromosome Aberrations, Forward Genetic Screens, and the Nature of Mutations

Genetics
Thomas C Kaufman

Abstract

The purpose of this chapter in FlyBook is to acquaint the reader with the Drosophila genome and the ways in which it can be altered by mutation. Much of what follows will be familiar to the experienced Fly Pusher but hopefully will be useful to those just entering the field and are thus unfamiliar with the genome, the history of how it has been and can be altered, and the consequences of those alterations. I will begin with the structure, content, and organization of the genome, followed by the kinds of structural alterations (karyotypic aberrations), how they affect the behavior of chromosomes in meiotic cell division, and how that behavior can be used. Finally, screens for mutations as they have been performed will be discussed. There are several excellent sources of detailed information on Drosophila husbandry and screening that are recommended for those interested in further expanding their familiarity with Drosophila as a research tool and model organism. These are a book by Ralph Greenspan and a review article by John Roote and Andreas Prokop, which should be required reading for any new student entering a fly lab for the first time.

References

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Citations

Feb 29, 2020·Genes·K M Tahsin Hassan Rahit, Maja Tarailo-Graovac
Mar 14, 2020·PLoS Genetics·Barbara FasuloNikolai Windbichler
Sep 19, 2018·Lab Animal·Douglas G HoweCynthia Smith
Mar 19, 2020·Frontiers in Physiology·Sylvia AntonFrédéric Marion-Poll
Sep 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ben Ewen-Campen, Norbert Perrimon
Aug 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Fillip PortMichael Boutros

Related Concepts

Metazoa
Autosome Abnormalities
Drosophila melanogaster
Miosis Disorder
History,20th Century
Historical Events,21st Century
Genome, Insect
Chromosomes
Drosophila
Genome

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