A short sequence within two purine-rich enhancers determines 5' splice site specificity.

Molecular and Cellular Biology
L L ElrickS M Berget

Abstract

Purine-rich enhancers are exon sequences that promote inclusion of alternative exons, usually via activation of weak upstream 3' splice sites. A recently described purine-rich enhancer from the caldesmon gene has an additional activity by which it directs selection of competing 5' splice sites within an alternative exon. In this study, we have compared the caldesmon enhancer with another purine-rich enhancer from the chicken cardiac troponin T (cTNT) gene for the ability to regulate flanking splice sites. Although similar in sequence and length, the two enhancers demonstrated strikingly different specificities towards 5' splice site choice when placed between competing 5' splice sites in an internal exon. The 32-nucleotide caldesmon enhancer caused effective usage of the exon-internal 5' splice site, whereas the 30-nucleotide cTNT enhancer caused effective usage of the exon-terminal 5' splice site. Both enhancer-mediated splicing pathways represented modulation of the default pathway in which both 5' splice sites were utilized. Each enhancer is multipartite, consisting of two purine-rich sequences of a simple (GAR)n repeat interdigitated with two enhancer-specific sequences. The entire enhancer was necessary for maximal splice ...Continue Reading

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Citations

Feb 27, 2001·Molecular and Cellular Endocrinology·J HanH Jarry
Apr 8, 2009·International Review of Cell and Molecular Biology·Jim Jung-Ching LinJian-Ping Jin
Jun 15, 2007·Nucleic Acids Research·Erming WangFranca Cambi
Jan 28, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·Makiko HoshiyaPatricia K Donahoe
Mar 12, 2002·The Journal of Biological Chemistry·Brandon J Biesiadecki, Jian-Ping Jin
May 25, 2002·Molecular and Cellular Biology·Martin J Simard, Benoit Chabot
Nov 30, 2000·Molecular and Cellular Biology·A J McCullough, S M Berget
Mar 21, 2003·The Journal of Biological Chemistry·Bianca J LamKlemens J Hertel
Aug 15, 2001·The Journal of Biological Chemistry·N YousafH Riedel
Sep 5, 2002·Molecular Genetics and Metabolism·Runu DeyCecile Marsac

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