A Short-Term Exposure to Tributyltin Blocks Leydig Cell Regeneration in the Adult Rat Testis

Frontiers in Pharmacology
Xiaolong WuRen-Shan Ge

Abstract

Background: Tributyltin (TBT) is widely used as an antifouling agent that may cause reproductive toxicity. The mechanism of TBT on Leydig cell development is still unknown. The objective of the present study was to investigate whether a brief exposure to low doses of TBT permanently affects Leydig cell development and to clarify the underlying mechanism. Methods: Adult male Sprague Dawley rats were randomly assigned into four groups and gavaged normal saline (control), 0.1, 1.0, or 10.0 mg/kg/day TBT for a consecutive 10 days, respectively. At the end of TBT treatment, all rats received a single intraperitoneal injection of 75 mg/kg ethane dimethane sulfonate (EDS) to eliminate all of adult Leydig cells. Leydig cells began a developmental regeneration process on post-EDS day 35. The Leydig cell regeneration was evaluated by measuring serum testosterone, luteinizing hormone, and follicle-stimulating hormone levels on post-EDS day 7, 35, and 56, the expression levels of Leydig cell genes, Leydig cell morphology and number and proliferation on post-EDS day 56. Results: TBT significantly reduced serum testosterone levels on post-EDS day 35 and 56 and increased serum luteinizing hormone and follicle-stimulating hormone levels on pos...Continue Reading

References

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Citations

May 25, 2020·Andrologia·Vaithinathan SelvarajuRalf Henkel
Dec 18, 2018·Frontiers in Pharmacology·Xiande HuangYongsheng Yin
Aug 28, 2019·International Journal of Toxicology·Derong MaGuoqiang Xue
Feb 19, 2021·Stem Cell Reviews and Reports·Zhuo-Jie LiuZhi-Jun Zang
Jun 7, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xingwang LiRen-Shan Ge
Mar 25, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Soudeh Ghafouri-FardMohammad Taheri
May 5, 2021·Expert Review of Molecular Diagnostics·Xu YouHaixia Zhao
Feb 2, 2022·Environmental Toxicology·Shijun LiRen-Shan Ge

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Methods Mentioned

BETA
Protein Assay
immunoprecipitation assay
chip
PCR

Software Mentioned

Pro Plus
GraphPad
GraphPad Prism
Image

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