PMID: 8593312Oct 1, 1995Paper

A short term in vitro cultivation of Babesia rodhaini and Babesia microti

The Journal of Veterinary Medical Science
S ShikanoK Ono

Abstract

In vitro cultivation of Babesia rodhaini (BR) and Babesia microti (BM) was attempted. When RPMI1640 was supplemented with 30 or 40% of non-treated fetal bovine serum (FBS), the gas mixture of 3% CO2-8% O2 best supported the growth of both parasites. Under this optimized condition, the percent parasitized erythrocytes peaked to approximately 4- and 2-times initial values for Br and BM, respectively. The cultivated parasites retained the infectivity to the host mice. BM showed the characteristic feature of division during cultivation. However, the lots of FBS will have to be taken into consideration, since the FBS lots were shown to give large varieties to the results. Selection of the appropriate FBS lot may yield the better growth of these protozoa.

Citations

Jul 5, 2002·Clinical Microbiology Reviews·Frederick L Schuster
May 29, 1998·International Journal for Parasitology·S ShikanoK Ono
Dec 20, 2012·The American Journal of Tropical Medicine and Hygiene·Mizuki SasakiHiromi Ikadai
Feb 14, 2020·Pathogens·Mingming LiuXuenan Xuan
Feb 17, 2019·Parasitology Research·Zetian ChenXiangye Liu

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