A short-term in vitro gill culture system to study the effects of toxic (copper) and non-toxic (cortisol) stressors on the rainbow trout, Oncorhynchus mykiss (Walbaum)

Toxicology in Vitro : an International Journal Published in Association with BIBRA
A F MazonS E Wendelaar Bonga

Abstract

A short-term (24 h) method of gill filament culture system was developed to predict the effects of environmental contamination and stress in fish. Gill culture system containing two or three rainbow trout gill filaments in sterile glutamine supplemented Leibovitz 15 (L-15) media was submitted for 24 h to six different treatments: (i) CONT (control, medium only); (ii) CORT (cortisol, 0.28 microM cortisol); (iii) BLOCK (glucocorticoid receptor blocker, 14 microM RU 486); (iv) CORT+BLOCK (cortisol and blocker, 0.28 microM cortisol+14 microM RU 486); (v) CORT+CU (cortisol and copper, 100 microM CuSO4+0.28 microM cortisol); (vi) CU (copper, 100 microM CuSO4). After 24 h, the overall gill structure and cellular components resembled those of salmonids in vivo. Lactate dehydrogenase (LDH) activity in the culture media increased in the CORT+CU and CU groups but was significantly lower in the CORT+CU compared to CU group. Apoptotic cells increased in the CORT and CORT+BLOCK. The numbers of glucocorticoid (GR) receptor-positive cells were lower in the CU group. This short-term culture system seems to be suitable for studying the effects of both external and internal stress effectors (toxicants and hormones respectively), as it contains al...Continue Reading

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Citations

Apr 21, 2011·Cell Biology and Toxicology·Isabelle LeguenPatrick Prunet
Sep 26, 2009·Toxicology Mechanisms and Methods·Piya KosaiKanitta Jiraungkoorskul
Dec 6, 2012·Ecotoxicology and Environmental Safety·M MelaH C Silva de Assis
Feb 8, 2011·Ecotoxicology and Environmental Safety·Peranandam RevathiNatesan Munuswamy
Nov 3, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·I LeguenP Prunet
Jun 1, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Cleoni Dos Santos Carvalho, Marisa Narciso Fernandes
Aug 21, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Cleverson Agner RamosWallice Paxiuba Duncan
Jul 16, 2015·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Hanna Kalamarz-KubiakEwa Kulczykowska
Dec 16, 2006·Toxicology·Aurélia de Fátima MazonMarisa Narciso Fernandes
Oct 12, 2010·Ecotoxicology and Environmental Safety·Sofia Garcia-SantosJ M Wilson
Dec 12, 2012·Archives of Environmental Contamination and Toxicology·Qi-Liang ChenYan-Hong Zhao
Sep 23, 2016·Journal of Exercise Rehabilitation·Yoon-Soo HanJi-Youn Kim
Jan 20, 2021·Zebrafish·Elizabeth E LeClair
Mar 31, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Alex M ZimmerChris N Glover

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