Abstract
A short-term (24 h) method of gill filament culture system was developed to predict the effects of environmental contamination and stress in fish. Gill culture system containing two or three rainbow trout gill filaments in sterile glutamine supplemented Leibovitz 15 (L-15) media was submitted for 24 h to six different treatments: (i) CONT (control, medium only); (ii) CORT (cortisol, 0.28 microM cortisol); (iii) BLOCK (glucocorticoid receptor blocker, 14 microM RU 486); (iv) CORT+BLOCK (cortisol and blocker, 0.28 microM cortisol+14 microM RU 486); (v) CORT+CU (cortisol and copper, 100 microM CuSO4+0.28 microM cortisol); (vi) CU (copper, 100 microM CuSO4). After 24 h, the overall gill structure and cellular components resembled those of salmonids in vivo. Lactate dehydrogenase (LDH) activity in the culture media increased in the CORT+CU and CU groups but was significantly lower in the CORT+CU compared to CU group. Apoptotic cells increased in the CORT and CORT+BLOCK. The numbers of glucocorticoid (GR) receptor-positive cells were lower in the CU group. This short-term culture system seems to be suitable for studying the effects of both external and internal stress effectors (toxicants and hormones respectively), as it contains al...Continue Reading
References
Mar 1, 1984·General and Comparative Endocrinology·T SandorA Z Mehdi
Feb 1, 1995·General and Comparative Endocrinology·Y IgerS E Wendelaar Bonga
Nov 1, 1994·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·L S OrtegoW H Benson
Dec 1, 1994·General and Comparative Endocrinology·P H BalmS E Bonga
Jul 1, 1997·Physiological Reviews·S E Wendelaar Bonga
Jun 1, 1997·Brain, Behavior, and Immunity·F A WeytsS E Wendelaar Bonga
May 8, 1998·General and Comparative Endocrinology·M TujagueB Ducouret
May 8, 1998·Biochemical and Biophysical Research Communications·Y KawaiI Miyamori
Jan 6, 2000·General and Comparative Endocrinology·L L CarruthD O Norris
May 10, 2000·Steroids·L ColombeY Guiguen
Sep 22, 2000·Aquatic Toxicology·Z C DangR A Lock
Mar 14, 2001·Developmental and Comparative Immunology·K J LaingC J Secombes
Mar 20, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·C M WoodP Pärt
Apr 20, 2001·The Journal of Endocrinology·C M ParianteA H Miller
May 23, 2001·Contraception·H B CroxattoH von Hertzen
Mar 19, 2002·Environmental Research·A F MazonM N Fernandes
Jun 1, 1996·Tissue & Cell·A K JulliardL Astic
Nov 1, 1994·Fish Physiology and Biochemistry·P LaurentJ Lignon
Citations
Apr 21, 2011·Cell Biology and Toxicology·Isabelle LeguenPatrick Prunet
Sep 26, 2009·Toxicology Mechanisms and Methods·Piya KosaiKanitta Jiraungkoorskul
Jan 15, 2013·Aquatic Toxicology·Ana LuzioAna M Coimbra
Dec 6, 2012·Ecotoxicology and Environmental Safety·M MelaH C Silva de Assis
Feb 8, 2011·Ecotoxicology and Environmental Safety·Peranandam RevathiNatesan Munuswamy
Nov 3, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·I LeguenP Prunet
Jun 1, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Cleoni Dos Santos Carvalho, Marisa Narciso Fernandes
Aug 21, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Cleverson Agner RamosWallice Paxiuba Duncan
Jul 16, 2015·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Hanna Kalamarz-KubiakEwa Kulczykowska
Dec 16, 2006·Toxicology·Aurélia de Fátima MazonMarisa Narciso Fernandes
Mar 28, 2006·Aquatic Toxicology·Alexandra GagnonAlice Hontela
Oct 12, 2010·Ecotoxicology and Environmental Safety·Sofia Garcia-SantosJ M Wilson
Nov 30, 2010·Life Sciences·Chiyo TakagiTatsuya Sakamoto
Mar 19, 2014·Marine Pollution Bulletin·Lourduraj Arockia VasanthiNatesan Munuswamy
Aug 20, 2016·Aquatic Toxicology·Erica K BrockmeierFrederic D L Leusch
Dec 22, 2011·Experimental Biology and Medicine·Shun LiY James Kang
Oct 13, 2006·Archives of Environmental Contamination and Toxicology·Na LiJian Yang
Dec 12, 2012·Archives of Environmental Contamination and Toxicology·Qi-Liang ChenYan-Hong Zhao
Sep 23, 2016·Journal of Exercise Rehabilitation·Yoon-Soo HanJi-Youn Kim
Aug 9, 2017·Ecotoxicology·Laura M LanganAwadhesh N Jha
Jan 20, 2021·Zebrafish·Elizabeth E LeClair
Mar 31, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Alex M ZimmerChris N Glover
Jan 21, 2006·The Journal of Experimental Biology·A F MazonM O Huising