A shotgun proteomic approach reveals novel potential salivary protein biomarkers for asthma.

The Journal of Asthma : Official Journal of the Association for the Care of Asthma
Orapan PoachanukoonSittichai Koontongkaew

Abstract

The aim of this study was to determine if there is an association between the salivary protein profile and disease control in asthma. Thirty asthmatic patients (17 adults and 13 children) participated in this study. Saliva samples were collected from healthy subjects, controlled and uncontrolled asthmatics. Individual samples from each group were combined to form a pooled sample, from which proteomic analysis was performed using gel-based quantitative proteomics. Fourteen out of thirty asthmatics were classified to be controlled asthma. Most of asthmatics received inhaled corticosteroids as the controller medications. SDS-PAGE showed predominant bands at high molecular weight in asthmatic saliva compared to that of the controls. Shotgun proteomic analyses indicated that 193 salivary proteins were expressed in both controlled and uncontrolled asthmatics. They were predicted to associate with proteins involved in pathogenesis of asthma including IL-5, IL-6, MCP-1, VEGF, and periostin and asthma medicines (Cromolyn, Nedocromil, and Theophylline). Nucleoside diphosphate kinase (NME1-NME2) only expressed in controlled asthmatics whereas polycystic kidney and hepatic disease 1 (PKHD1)/fibrocystin, zinc finger protein 263 (ZNF263), un...Continue Reading

References

Nov 6, 2003·American Journal of Physiology. Renal Physiology·Lisa M Guay-Woodford
Oct 14, 2006·Journal of Bioenergetics and Biomembranes·Richmond MuimoAnil Mehta
Jan 9, 2007·The Journal of Allergy and Clinical Immunology·Zheng YangCarolyn L Geczy
Aug 21, 2007·Expert Review of Proteomics·Shen HuDavid T Wong
Dec 18, 2007·Nucleic Acids Research·Michael KuhnPeer Bork
Oct 22, 2009·Physiological Genomics·Anurag AgrawalBalaram Ghosh
Nov 10, 2009·Canadian Journal of Physiology and Pharmacology·Andrew J Halayko, Saeid Ghavami
May 17, 2011·Omics : a Journal of Integrative Biology·Sandra K Al-TarawnehSompop Bencharit
Jul 15, 2011·The Journal of Allergy and Clinical Immunology·Chang XiaoDonna E Davies
Jul 22, 2011·Archives of Oral Biology·Sausan Al KawasDavid B Ferguson
May 2, 2012·BMC Bioinformatics·Mark R DalmanZhong-Hui Duan
Sep 7, 2013·Biologics : Targets & Therapy·Susanne Jh VijverbergLeo Koenderman
Sep 10, 2013·American Journal of Respiratory Cell and Molecular Biology·Dinesh K PillaiMary C Rose
Sep 24, 2013·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Tae-Hyeong LeeChoon-Sik Park
Apr 25, 2014·Allergology International : Official Journal of the Japanese Society of Allergology·Hisako Matsumoto
Nov 5, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Qiaolin HuXing-Zhen Chen
Feb 27, 2015·International Journal of Epidemiology·Vania JanuarJoanne Ryan
Apr 20, 2012·Journal of Allergy & Therapy·Blanca Camoretti-MercadoMarion A Hofmann Bowman
Nov 22, 2015·Nucleic Acids Research·Damian SzklarczykMichael Kuhn
Dec 3, 2016·Journal of Applied Physiology·Douglas Curran-Everett
Jan 28, 2017·Journal of Investigational Allergology & Clinical Immunology·A Habernau MenaP Bobadilla González
Dec 5, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Paul V Attwood, Richmond Muimo
Mar 15, 2018·BMC Oral Health·Sineepat TalungchitSiribang-On Piboonniyom Khovidhunkit
Jun 14, 2018·Journal of Applied Oral Science : Revista FOB·Talita Mendes da Silva VenturaMarília Afonso Rabelo Buzalaf
Jun 16, 2018·Biochemical and Biophysical Research Communications·Karam KimIl Yup Chung
Jan 1, 2020·Journal of Biological Research·Katerina R Katsani, Dimitra Sakellari

❮ Previous
Next ❯

Citations

May 1, 2021·International Journal of Molecular Sciences·Emanuela di PalmoAndrea Pession
Aug 9, 2021·Molecular Aspects of Medicine·Zsolt I KomlósiCezmi A Akdis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.