A sialylation study of mouse brain gangliosides by MALDI a-TOF and o-TOF mass spectrometry

Journal of Mass Spectrometry : JMS
Mostafa ZareiJasna Peter-Katalinić

Abstract

Matrix-assisted laser desorption/ionization (MALDI) process of sialoglycoconjugates is generally accompanied by different levels of cleavage of sialic acid residues and/or by dehydration, and decarboxylation reactions. Quantitative densitometry of the mouse brain ganglioside (MBG) components separated by high-performance thin layer chromatography (HPTLC) and evidenced by orcinol staining was a basis to verify the ganglioside composition pattern with respect to the relative abundances of individual components in the mixture. A systematic mass spectrometry (MS) sialylation analysis has been carried out to evaluate the feasibility of an axial time-of-flight (a-TOF) MS, equipped with a vacuum MALDI source and an orthogonal-TOF (o-TOF) instrument with an ion source operated at about 1 mbar of N(2). Besides, the esterification by one methyl group of the carboxyl group in sialic acid to increase the stability of the ganglioside species for MALDI MS analysis has been tested and the yield of intact ganglioside species and of the neutral loss of water and carbon dioxide estimated. For the sialylation analysis of native ganglioside mixtures the MALDI o-TOF analysis with 6-azo-2-thiothymine/diammonium citrate (ATT/DAC) as a matrix appears ...Continue Reading

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Citations

Aug 4, 2010·Neuromolecular Medicine·Yanfeng ChenAlfred H Merrill
Jan 21, 2012·Journal of Lipid Research·Alicia L RichardsSarah Trimpin
Jul 26, 2013·Critical Reviews in Biochemistry and Molecular Biology·Sarah TrimpinEllen D Inutan
Nov 30, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Takashi NishikazeJunko Amano
Nov 11, 2009·Rapid Communications in Mass Spectrometry : RCM·Takashi Nishikaze, Junko Amano
Apr 7, 2009·Analytica Chimica Acta·Kenneth ChanJianjun Li
Aug 25, 2015·Journal of Pharmaceutical and Biomedical Analysis·Maria CondelloStefania Meschini
Nov 30, 2010·The Analyst·Shelley N JacksonAmina S Woods
Jan 1, 2012·Metabolites·Hany Farwanah, Thomas Kolter
Aug 16, 2019·Cells·Qiongyu LiCarlito B Lebrilla
Mar 20, 2018·Journal of the American Society for Mass Spectrometry·Shelley N JacksonAmina S Woods

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