PMID: 2107551Mar 1, 1990Paper

A signaling role for the cytoplasmic segment of the CD8 alpha chain detected under limiting stimulatory conditions

Proceedings of the National Academy of Sciences of the United States of America
F LetourneurB Malissen

Abstract

To test for the functional importance of the cytoplasmic segment of the CD8 molecule, a mouse T-cell hybridoma expressing a T-cell receptor specific for the class I major histocompatibility complex product H-2Kb was transfected with a set of CD8 alpha-chain (Ly-2) and/or beta-chain (Ly-3) genes encoding polypeptides with carboxyl-terminal truncations or substitutions. When challenged with Kb-positive splenocytes, transfectants expressing Ly-2 homodimers that lacked cytoplasmic tails responded nearly as effectively as wild-type Ly-2 transfectants. However in marked contrast to the wild-type Ly-2 transfectants, tailless Ly-2 transfectants were greatly impaired in their ability to respond to Kb-transfected L cells. Coexpression of the Ly-3 gene did not restore this impaired response. The unique functional property of the Ly-2 alpha cytoplasmic segment was further supported by the analysis of a chimeric Ly-3 subunit in which the cytoplasmic segment was replaced by the one from the Ly-2 alpha subunit. When associated with a soluble Ly-2 subunit lacking a transmembrane segment, the chimeric Ly-3 was indeed sufficient to restore the response to Kb-transfected L cells. Since the lateral mobility of the tailless Ly-2 molecules on the ce...Continue Reading

References

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Citations

Aug 23, 1993·Biochimica Et Biophysica Acta·C E RuddM Yamamoto
Jan 1, 1992·Biophysical Journal·O TissotP Bongrand
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M C MiceliJ R Parnes
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May 21, 1992·Nature·C J WheelerJ R Parnes
Apr 17, 2003·The Journal of Biological Chemistry·Sarah L HutchinsonAndrew K Sewell
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Jun 11, 2014·Cancer Immunology Research·Dinesh ChandraClaudia Gravekamp
Jun 3, 2021·International Journal of Molecular Sciences·Omid Sascha YousefiWolfgang W A Schamel

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