A silent allele in the locus D19S433 contained within the AmpFlSTR Identifiler PCR Amplification Kit

Legal Medicine
Akiko TsujiNoriaki Ikeda

Abstract

We present two cases where a single locus mismatch was found in the locus D19S433 using the AmpFlSTR Identifiler PCR Amplification Kit (Applied Biosystems) (Identifiler Kit) during paternity and maternity tests. This mismatch differed from the mismatch pattern where there is usually a one repeat difference. We designed forward and reverse primers so that they were positioned further away from the primer set contained in the Identifiler Kit. The results showed the existence of a silent allele 13 in both families, due to a point mutation that changed guanine to adenine at 32 nucleotides downstream from the 3' end of the AAGG repeat sequences in all four members. A single locus mismatch due to a silent allele may occur in any locus using any kit. Accordingly, we should pay attention to this silent allele when carrying out human identification and parentage analysis.

References

Mar 26, 2004·Forensic Science International : Synergy·T M ClaytonA J Urquhart
Apr 5, 2007·International Journal of Legal Medicine·Nancy VanderheydenRonny Decorte
Jun 15, 2007·Legal Medicine·Tomohiro TakayamaYoshihisa Watanabe
Jul 19, 2008·Journal of Forensic Sciences·Natsuko MizunoKentaro Kasai

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Citations

Oct 12, 2013·Legal Medicine·Munetaka Ozeki, Keiji Tamaki
Mar 25, 2014·Annals of Human Biology·Wenjing ChenHongyu Sun
May 6, 2011·Forensic Science International. Genetics·Hang Yee WongWai Fun Tan-Siew
Dec 3, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Michele Curcio, Silvano Presciuttini
Dec 15, 2020·Journal of Forensic Sciences·Hagit SchayekOrna Avlas
Jul 25, 2021·Molecular Genetics & Genomic Medicine·Chengchen ShaoKuan Sun

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