A Silk Fibroin/Collagen Nerve Scaffold Seeded with a Co-Culture of Schwann Cells and Adipose-Derived Stem Cells for Sciatic Nerve Regeneration

PloS One
Yunqiang XuShiqing Feng

Abstract

As a promising alternative to autologous nerve grafts, tissue-engineered nerve grafts have been extensively studied as a way to bridge peripheral nerve defects and guide nerve regeneration. The main difference between autogenous nerve grafts and tissue-engineered nerve grafts is the regenerative microenvironment formed by the grafts. If an appropriate regenerative microenvironment is provided, the repair of a peripheral nerve is feasible. In this study, to mimic the body's natural regenerative microenvironment closely, we co-cultured Schwann cells (SCs) and adipose-derived stem cells (ADSCs) as seed cells and introduced them into a silk fibroin (SF)/collagen scaffold to construct a tissue-engineered nerve conduit (TENC). Twelve weeks after the three different grafts (plain SF/collagen scaffold, TENC, and autograft) were transplanted to bridge 1-cm long sciatic nerve defects in rats, a series of electrophysiological examinations and morphological analyses were performed to evaluate the effect of the tissue-engineered nerve grafts on peripheral nerve regeneration. The regenerative outcomes showed that the effect of treatment with TENCs was similar to that with autologous nerve grafts but superior to that with plain SF/collagen sc...Continue Reading

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Jun 2, 2018·Journal of Neural Engineering·Anjali Vijay DhobaleD Kacy Cullen
Jan 14, 2018·Scientific Reports·Elena StoccoClaudio Grandi
Jun 15, 2018·Journal of Biomedical Materials Research. Part a·Maryam Nazm BojnordiHatef Ghasemi Hamidabadi
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Jul 6, 2019·ACS Chemical Neuroscience·Shadi HoushyarRobert Shanks

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Methods Mentioned

BETA
flow cytometry
Scanning electron microscopy

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