PMID: 2500920Jan 1, 1989Paper

A similar protein portion for two exoglucanases secreted by Saccharomyces cerevisiae

Archives of Microbiology
M RamírezG Larriba

Abstract

Exoglucanase (exo-1,3-beta-D-glucan glycohydrolase, EC 3.2.1.56) activity secreted by Saccharomyces cerevisiae into the culture medium was separated by ion exchange chromatography into two glycoprotein isoenzymes which contributed 10% (exoglucanase I) and 90% (exoglucanase II) towards the total activity. Analysis of the "in vitro" deglycosylated products by polyacrylamide gel electrophoresis under native or denaturing conditions indicated that the protein portions of both exoglucanases exhibited identical mobility, each one consisting of two polypeptides with Mr of 47,000 and 48,000. The same profile was shown by the exoglucanase secreted in the presence of tunicamycin. Antibodies raised against the protein portion of exoglucanase II did react with both native exoglucanases and their deglycosylated products with a pattern indicative of immunological identity. Digestion of the "in vitro" deglycosylated products of both exoglucanases with Staphylococcus aureus V-8 protease or trypsin generated the same proteolytic fragments in each case. Only exoglucanase II was secreted by protoplasts. These and previously reported results indicate that the protein portions of both isoenzymes may be the product of the same gene (or a family of r...Continue Reading

References

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Feb 24, 1989·Biochimica Et Biophysica Acta·M RamírezG Larriba
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Citations

Aug 21, 2013·Current Genetics·R Teparić, Vladimir Mrsa
Dec 8, 1989·Biochimica Et Biophysica Acta·J C RidruejoG Larriba
Jan 15, 1995·FEMS Microbiology Letters·G LarribaR D Basco
Aug 9, 2005·International Journal of Food Microbiology·José V GilLuis González-Candelas
Jun 26, 2013·Journal of Agricultural and Food Chemistry·Chun-Hui ChiuTing-Jang Lu
Oct 1, 1995·The Journal of Cell Biology·E Harsay, A Bretscher
Jan 11, 2011·Applied and Environmental Microbiology·Sabine SchmidtStefan Martens
May 1, 1991·Yeast·J G De Nobel, J A Barnett
Jul 29, 2004·The Journal of Biological Chemistry·Raúl CondeGermán Larriba
Sep 1, 1995·Microbiological Reviews·V J CidM Sánchez

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