A simple, accurate, time-saving and green method for the determination of 15 sulfonamides and metabolites in serum samples by ultra-high performance supercritical fluid chromatography

Journal of Chromatography. a
Yuan ZhangFeng Zhang

Abstract

An analytical method based on ultra-high performance supercritical fluid chromatography (UHPSFC) with photo-diode array detection (PDA) has been developed to quantify 15 sulfonamides and their N4-acetylation metabolites in serum. Under the optimized gradient elution conditions, it took only 7min to separate all 15 sulfonamides and the critical pairs of each parent drug and metabolite were completely separated. Variables affecting the UHPSFC were optimized to get a better separation. The performance of the developed method was evaluated. The UHPSFC method allowed the baseline separation and determination of 15 sulfonamides and metabolites with limit of detection ranging from 0.15 to 0.35μg/mL. Recoveries between 90.1 and 102.2% were obtained with satisfactory precision since relative standard deviations were always below 3%. The proposed method is simple, accurate, time-saving and green, it is applicable to a variety of sulfonamides detection in serum samples.

References

May 26, 2012·Mini Reviews in Medicinal Chemistry·Syed Shoaib Ahmad ShahMuhammad Ashfaq
Dec 3, 2014·Analytica Chimica Acta·Stanislava G DmitrienkoYury A Zolotov

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Citations

Mar 1, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenhua LiWen-Chen Suen
Feb 21, 2018·The Analyst·H Kočová VlčkováL Nováková
Aug 10, 2021·Journal of the American Society for Mass Spectrometry·Yaju ZhaoDanke Xu

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