A Simple and Effective Sample Preparation Strategy for MALDI-MS Imaging of Neuropeptide Changes in the Crustacean Brain Due to Hypoxia and Hypercapnia Stress.

Journal of the American Society for Mass Spectrometry
Amanda R BuchbergerLingjun Li

Abstract

Matrix-assisted laser desorption/ionization (MALDI)-MS imaging has been utilized to image a variety of biomolecules, including neuropeptides. Washing a tissue section is an effective way to eliminate interfering background and improve detection of low concentration target analyte molecules; however, many previous methods have not been tested for neuropeptide analysis via MALDI-MS imaging. Using crustaceans as a neurological model organism, we developed a new, simple washing procedure and applied this method to characterize neuropeptide changes due to hypoxia stress. With a 10 s 50:50 EtOH:H2O wash, neuropeptide coverage was improved by 1.15-fold, while normalized signal intensities were increased by 5.28-fold. Specifically, hypoxia and hypercapnia stress conditions were investigated due to their environmental relevance to marine invertebrates. Many neuropeptides, including RFamides, pyrokinin, and cardioactive peptides, showed distinct up- and down-regulation for specific neuropeptide isoforms. Since crustacean neuropeptides are homologous to those found in humans, results from these studies can be applied to understand potential roles of neuropeptides involved in medical hypoxia and hypercapnia.

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Citations

Nov 10, 2020·Mass Spectrometry Reviews·Kellen DeLaneyLingjun Li
Nov 21, 2020·Analytical and Bioanalytical Chemistry·Qiuqin ZhouCarsten Hopf
Aug 11, 2021·Expert Review of Proteomics·Christopher S SauerLingjun Li
Aug 14, 2020·Journal of Proteome Research·Fernando Tobias, Amanda B Hummon
Aug 27, 2021·Journal of the American Society for Mass Spectrometry·Jarod A FincherJeffrey M Spraggins
Sep 25, 2021·Mass Spectrometry Reviews·Ashley PhetsanthadLingjun Li
Nov 18, 2020·Current Protein & Peptide Science·Nhu Q VuLingjun Li

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