PMID: 39323Jul 20, 1979

A simple and efficient method for preparation of immunoelectrophoretically pure guinea pig IgM and isolation of monospecific anti-IgM antibodies

Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie
H Mauch, G Kümel

Abstract

This report describes experimental conditions developed for the rapid and efficient preparation of guinea pig IgM in a purified form and in high yield. A combined immunization procedure had to be designed to obtain sufficiently high IgM concentrations in guinea pig serum. The isolation procedure includes precipitation at low pH, followed by sedimentation at 110,000 g and reverse flow gel chromatography on Sephades G-200. Furthermore,we describe in detail the isolation of relatively large amounts of pure and specific anti-guinea pig mu-chain antibodies by immunoaffinity chromatography.

References

Apr 21, 1979·Journal of Chromatography·G KümelH Mauch
Jan 1, 1979·International Archives of Allergy and Applied Immunology·H Mauch, G Kümel
Dec 1, 1970·The Biochemical Journal·R G Leslie, S Cohen
Feb 1, 1971·European Journal of Biochemistry·R Axén, S Ernback
Feb 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·R A Reisfeld, N E Hyslop

Related Concepts

Anti-Antibodies
Antibody Formation
Cavia porcellus
Hydrogen-Ion Concentration
IgM2
Immunoglobulin mu-Chains

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