PMID: 9163993May 1, 1996Paper

A simple and inexpensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp

Revista Do Instituto De Medicina Tropical De São Paulo
F Hernández

Abstract

Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x 8 in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories.

References

Mar 1, 1992·Revista Do Instituto De Medicina Tropical De São Paulo·F Hernández, P Rivera
Jan 1, 1989·Annual Review of Medicine·T L Cover, M J Blaser
Sep 1, 1987·American Journal of Epidemiology·M S DemingR O Sikes
Apr 1, 1986·American Journal of Public Health·N V HarrisC M Nolan
Dec 30, 1983·The American Journal of Medicine·H Kaplan

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Citations

Feb 27, 2007·International Journal of Food Microbiology·Y GhafirG Daube
Sep 19, 2019·História, ciências, saúde--Manguinhos·Elaine da Silveira Leite

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