A simple and quick method to concentrate MSCV retrovirus

Blood Cells, Molecules & Diseases
Eiki Kanbe, Dong-Er Zhang

Abstract

Retroviral-mediated gene transfer is widely used to express proteins in hematopoietic cells for the analysis of their effects on blood cell proliferation, differentiation, and biological function. The efficiency of gene transfer depends on the concentration of retrovirus in cell culture medium during infection. Here, we report one simple and quick method to increase titers of retrovirus. Centrifugation of murine stem cell virus (MSCV) in either microcentrifuge or high-speed centrifuge for only 1 h can significantly concentrate retrovirus at the bottom of the tube. The resuspended retrovirus can be used effectively to infect target cells in gene transfer experiments.

References

Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W S PearD Baltimore
Aug 1, 1995·Journal of Virological Methods·A B BahnsonJ A Barranger

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Citations

Sep 21, 2012·Nature·Roxane TussiwandKenneth M Murphy
Sep 1, 2012·Nature Protocols·Alison M Karst, Ronny Drapkin
Apr 12, 2012·Molecular Biotechnology·Rosalinda MadonnaRaffaele De Caterina
May 10, 2005·Arthritis and Rheumatism·Mariano Vega-OstertagSilvia S Pierangeli
Jun 10, 2015·Nature Immunology·Gary E Grajales-ReyesKenneth M Murphy

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