PMID: 8946015Nov 13, 1996Paper

A simple and rapid method for removal of specific cell populations from whole blood

Journal of Immunological Methods
R K ZwernerT R Russell

Abstract

A method is presented in which specific cells can be rapidly separated from whole blood by the use of antibody labelled dense particles utilizing gravity as a means of separation. This method specifically removes the targeted cells with minimal non-specific cell loss. The method is simple to perform and rapid, requiring less than 10 min for cell/particle binding and separation. The method is robust so that exact ratios of particles to cells are not needed. This method could be used in any separation scheme to remove specific cell populations without significant modification.

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L J Wysocki, V L Sato
Jan 1, 1990·Cytometry·S MiltenyiA Radbruch
Jul 11, 1986·Journal of Immunological Methods·R J BerensonD Kalamasz
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg

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Citations

Jan 22, 2004·Archives of Medical Research·Albertus W WognumTerry E Thomas
Mar 19, 1999·Methods : a Companion to Methods in Enzymology·T E ThomasC J Eaves

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