PMID: 6405785May 26, 1983Paper

A simple and rapid method for the preparation of plasma membranes

Biochimica Et Biophysica Acta
T MaedaS Q Mehdi

Abstract

A simple and rapid method for preparing plasma membranes from isolated cells or tissues is described. The membranes were characterised (a) biochemically by an analysis of specific marker enzymes, (b) by quantitation of cell surface receptors, and (c) immunologically by their ability to elicit specific allogeneic responses from cytotoxic T cells in secondary in vitro stimulations. Based on both biochemical and immunologic criteria, plasma membranes prepared by the method described here are of equal or greater 'purity' compared to those prepared by two other methods that are most widely used to date and the yields are several-fold higher.

References

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Citations

Dec 1, 1991·Journal of Bioenergetics and Biomembranes·I IbarrolaM Trueba
Oct 1, 1990·Biulleten' eksperimental'noĭ biologii i meditsiny·Z Sh SamagulovaE L Zumerov
Mar 1, 1991·The Journal of Membrane Biology·M TruebaJ M Macarulla
Oct 16, 1995·European Journal of Pharmacology·M MizunoH Koike
Sep 26, 1996·European Journal of Pharmacology·A I VallejoG Burnstock
Mar 16, 1988·Journal of Immunological Methods·M R WilsonR L Raison
May 1, 1990·The Journal of Nutritional Biochemistry·M Sigman, B Lönnerdal
Aug 31, 2002·Cellular Immunology·Jacqueline M McBrideGregorio Aversa
Aug 8, 1998·Virus Research·K B Andersen
Jan 8, 1999·Virus Research·K B Andersen, K E Olsen
Jul 4, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J P Zappulla, L DesGroseillers
Jun 8, 2001·European Journal of Biochemistry·O J CorderoF J Rodríguez-Berrocal
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A A Brian
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P McCafferyU C Dräger
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J PunnonenJ E de Vries
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraY Okada
Aug 1, 1990·Photochemistry and Photobiology·F P GasparroR L Edelson
Mar 16, 2006·Journal of Virology·Ramon MendozaJulie Overbaugh
Oct 1, 1992·American Journal of Respiratory Cell and Molecular Biology·M F BeersA B Fisher
Mar 1, 1994·The Journal of Clinical Investigation·C P KellyC Pothoulakis

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