A simple and rapid procedure for the purification of synthetic polypeptides by a combination of affinity chromatography and methionine chemistry

FEBS Letters
M A RoggeroG Corradin

Abstract

Chemical synthesis of bioactive peptides has become a widespread and rapidly growing technique due to automated and efficient protocols for chain assembly. For most applications, the crude synthetic product must be purified to remove residual reactants, failure sequences and chemically modified peptide species. We propose here a method of universal applicability based on immobilized metal ion affinity chromatography, CNBr cleavage and use of reversible Met-sulfoxide protection. With this method we were able to purify to homogeneity in high yield the PbCS 242-310 polypeptide corresponding to the C-terminal region of Plasmodium berghei CS protein.

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D E KriegerR B Merrifield
Nov 1, 1992·International Journal of Peptide and Protein Research·H L Ball, P Mascagni
Sep 1, 1991·Scandinavian Journal of Immunology·L R HaaheimA R Coates
Sep 1, 1991·International Journal of Peptide and Protein Research·G LindebergA Engström
Feb 1, 1990·Molecular and Biochemical Parasitology·D E Lanar
Jan 1, 1983·Methods in Enzymology·R A Houghten, C H Li
Aug 1, 1994·International Journal of Peptide and Protein Research·D D SmithB J Morley

❮ Previous
Next ❯

Citations

Jan 13, 1999·Applied Biochemistry and Biotechnology·C Rais-BeghdadiC D Reymond
Mar 8, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Riyasat AliD N Rao
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·R Zusman, I Zusman
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·I Zusman
Sep 24, 2010·Science Translational Medicine·Giampietro CorradinAntonio Verdini
Jan 14, 2009·Infection and Immunity·Aissatou Toure-BaldePierre Druilhe
Jun 4, 2013·Clinical and Translational Allergy·Céline PellatonFrançois Spertini
Apr 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C Von GarnierF Spertini
Jan 9, 2007·Experimental Parasitology·Slavica MasinaNicolas Fasel
Feb 14, 2006·Molecular Immunology·S PratoJ A Lopez
Oct 6, 2004·Expert Opinion on Biological Therapy·Giampietro CorradinAntonio Verdini
Feb 3, 2005·European Journal of Immunology·Sandro PratoJosé Alejandro López
Oct 10, 2006·Clinical and Vaccine Immunology : CVI·Sima RafatiBarbara Papadopoulou
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M AstoriF Spertini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.