PMID: 8586060Oct 1, 1995Paper

A simple and rapid technique for postelectrophoretic detection of proteases using azocasein

Electrophoresis
D Vázquez Peyronel, A M Cantera

Abstract

Azocasein, diffused into gels after polyacrylamide gel electrophoresis, provides a useful substrate for simple and rapid protease detection. After electrophoresis, the gels are incubated in an azocasein solution, immersed in trichloroacetic acid and then treated with NaOH. Proteolytic zones appear as uncolored bands on an orange background. The gels can be stored in trichloroacetic acid for months. This technique gives good results with serine proteases, acid proteases and metalloproteases. The method can be used under nondenaturing conditions and in the presence of sodium dodecyl sulfate. The sensitivity of this method is at the submicrogram protein level for trypsin.

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Citations

Apr 2, 2014·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Quanju ZhaoJianping Xie
Jul 21, 2004·Fitoterapia·Susana R MorcelleNora Priolo

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