A simple and reliable method to conduct and monitor expression cassette integration into the Escherichia coli chromosome

Biotechnology Journal
C AlbermannG A Sprenger

Abstract

We report a method for the integration of expression cassettes into the Escherichia coli chromosome using rare and dispensable sugar degradation gene loci as sites for integration. Clones carrying successfully recombined DNA fragments in the chromosome are easily screened using a solid differential medium containing the respective sugar compound. As an example for the heterologous expression of a complex natural product biosynthesis pathway, we show the stepwise chromosomal integration of the zeaxanthin biosynthesis pathway from Pantoea ananatis into E. coli.

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Citations

Dec 1, 2011·Applied Microbiology and Biotechnology·Daisuke KomaKiyofumi Sakai
Jan 20, 2011·Biotechnology Letters·Jiuzhou ChenXiaoyuan Wang
Apr 28, 2011·Microbial Cell Factories·Karin LemuthChristoph Albermann
Apr 14, 2016·Journal of Microbiological Methods·Shalini S DebArvind M Lali
Dec 24, 2014·Journal of Industrial Microbiology & Biotechnology·Xi-Ran LiJian-Zhong Liu
Oct 3, 2014·FEMS Microbiology Letters·Christoph AlbermannGeorg A Sprenger
May 27, 2017·Journal of Industrial Microbiology & Biotechnology·Shamlan M S ReshamwalaArvind M Lali
Jul 22, 2014·Chembiochem : a European Journal of Chemical Biology·Florian BaumgärtnerChristoph Albermann
Sep 11, 2018·Biotechnology and Bioengineering·Julia TröndleDirk Weuster-Botz
May 13, 2018·Journal of Industrial Microbiology & Biotechnology·Jin-Ying GuoXue-Li Zhang

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