A simple and sensitive assay for 25-hydroxyvitamin D, 24,25-dihydroxyvitamin D and 1,25-dihydroxyvitamin D in human serum

Clinica Chimica Acta; International Journal of Clinical Chemistry
M ImawariT Osuga

Abstract

An improved method is described which permits the simultaneous determination of 25-hydroxyvitamin D [25-(OH)D], 24,25-dihydroxyvitamin D [24,25-(OH)2D] and 1,25-dihydroxyvitamin D [1,25-(OH)2D] in milliliters of human serum. Methodological improvements enabled a rapid and almost complete extraction of the three metabolites from serum and omission of adding labeled internal standards to each serum sample for the calculation of individual recoveries. Commercially available stable chick embryo intestinal mucosa cytosol preparation made the troublesome preparation of cytosol receptor for 1,25-(OH)2D unnecessary. The procedure involves saturation of serum with ammonium carbonate and extraction with methanol/ethyl acetate, followed by separation of 25-(OH) D from the dihydroxy metabolites of vitamin D by Sephadex LH-20 column chromatography and further separation of the dihydroxy metabolites into 24,25-(OH)2D and 1,25-(OH)2D by high-pressure liquid chromatography. This is followed by individual determination of each metabolite by competitive protein-binding assay or radioreceptor assay.

References

Mar 1, 1977·The Journal of Clinical Investigation·M Imawari, D S Goodman
Mar 1, 1980·The Journal of Clinical Endocrinology and Metabolism·S LawoyinC Y Pak
Oct 1, 1980·The Journal of Clinical Endocrinology and Metabolism·L Aksnes, D Aarskog
Aug 10, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·M T ParviainenJ K Visakorpi
Mar 1, 1980·Clinics in Endocrinology and Metabolism·E B Mawer
Jan 1, 1981·Journal of Steroid Biochemistry·D A SeamarkH L Makin

❮ Previous
Next ❯

Citations

May 1, 1984·Digestive Diseases and Sciences·K KozawaY Morioka
Oct 15, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·S ScharlaE Mayer
Oct 31, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·A P De Leenheer, R M Bauwens
Jul 30, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·M BlayauP Peron
Oct 29, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·S WildermuthJ L O'Riordan
Dec 1, 1987·Journal of Steroid Biochemistry·C E PorteousH L Makin
Aug 8, 2019·International Journal of Molecular Sciences·Shaima SirajudeenAsma Al Menhali
Jul 29, 1988·Journal of Chromatography·A P De LeenheerR M Bauwens

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.