A simple and universal enzyme-free approach for the detection of multiple microRNAs using a single nanostructured enhancer of surface plasmon resonance imaging

Analytical and Bioanalytical Chemistry
Andrea SguasseroRenzo Vanna

Abstract

Here we describe a simple approach for the simultaneous detection of multiple microRNAs (miRNAs) using a single nanostructured reagent as surface plasmon resonance imaging (SPRi) enhancer and without using enzymatic reactions, sequence specific enhancers or multiple enhancing steps as normally reported in similar studies. The strategy involves the preparation and optimisation of neutravidin-coated gold nanospheres (nGNSs) functionalised with a previously biotinylated antibody (Ab) against DNA/RNA hybrids. The Ab guarantees the recognition of any miRNA sequence adsorbed on a surface properly functionalised with different DNA probes; at the same time, gold nanoparticles permit to detect this interaction, thus producing enough SPRi signal even at a low ligand concentration. After a careful optimisation of the nanoenhancer and after its characterisation, the final assay allowed the simultaneous detection of four miRNAs with a limit of detection (LOD) of up to 0.5 pM (equal to 275 attomoles in 500 μL) by performing a single enhancing injection. The proposed strategy shows good signal specificity and permits to discriminate wild-type, single- and triple-mutated sequences much better than non-enhanced SPRi. Finally, the method works p...Continue Reading

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Citations

Nov 25, 2019·Mikrochimica Acta·Farzaneh GhorbaniMehdi Yousefi
Aug 27, 2019·Frontiers in Chemistry·Noemi BellassaiGiuseppe Spoto
Feb 3, 2019·Analytical and Bioanalytical Chemistry·Roberta D'Agata, Giuseppe Spoto
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Sep 16, 2020·Biosensors & Bioelectronics·Asiyeh JebelliMiguel de la Guardia
May 3, 2021·Nano Convergence·Despina P Kalogianni
Aug 15, 2021·Biosensors & Bioelectronics·Jia-Huan QuDragana Spasic

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