A simple assay for optimizing yeast-mammalian cell fusion conditions

Molecular Biotechnology
David Markie

Abstract

Polyethylene glycol (PEG)-induced cell fusion can be a useful method for the transfer of yeast artificial chromosomes (YACs) from yeast spheroplasts to mammalian cells in culture, although success varies between recipient cell types. Experiments aimed at determining optimum fusion conditions can also be very time-consuming. To minimize this difficulty, a reporter plasmid has been constructed that allows yeast-mammalian cell fusion rates to be determined within 3 d. The speed and sensitivity of the assay should allow a more systematic evaluation of cell lines for their capacity to fuse with yeast, and for rapid optimization of fusion parameters.

References

May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Z LarinH Lehrach
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K R PetersonG Stamatoyannopoulos
Mar 1, 1993·Somatic Cell and Molecular Genetics·D MarkieW F Bodmer

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Citations

Jun 7, 2005·Biochemical and Biophysical Research Communications·Xu-Feng ZhangGuo-Xiang Cheng
Jan 19, 2020·Experimental Cell Research·David M Brown, John I Glass

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