A simple assay to identify peroxisomal proteins involved in 12-oxo-phytodienoic acid metabolism

Plant Signaling & Behavior
Gaëlle Cassin-Ross, Jianping Hu

Abstract

Peroxisomes are vital eukaryotic organelles that house a variety of metabolic functions. To fully define the proteome of plant peroxisomes, we recently performed a proteomic analysis of peroxisomes from etiolated Arabidopsis seedlings, verified the peroxisomal localization of candidate proteins by in vivo targeting analysis of fluorescent proteins, and subjected the T-DNA mutants of the newly confirmed proteins to a series of phenotypic analysis. Our reverse genetics approach revealed the role of a cysteine protease - Response to Drought 21A-like Cysteine Protease1 (RDL1) - in seed germination, indole-3-butyric acid (IBA) β-oxidation and stress response. Here, we developed a quick assay aimed at identifying peroxisomal proteins involved in the metabolism of 12-oxo-phytodienoic acid (OPDA), which is converted to jasmonic acid in the peroxisome through β-oxidation. We performed a survey of the same mutants analyzed in our previous reverse genetics study with this new assay by measuring the response of mutants to OPDA's inhibitory effect on root elongation. Mutants of RDL1 and SERINE CARBOXYPEPTIDASE-LIKE20 (SCPL20) exhibited statistically significant hypersensitivity to OPDA, indicating the potential involvement of these proteins...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·H van den BoschJ M Tager
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P E StaswickS H Howell
Sep 12, 2006·The Journal of Biological Chemistry·Abraham J K KooGregg A Howe
Jan 1, 2009·The Arabidopsis Book·Navneet KaurJianping Hu
Jan 1, 2010·The Arabidopsis Book·Iván F Acosta, Edward E Farmer
Jun 7, 2012·The Plant Cell·Jianping HuBethany K Zolman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.