A simple colorimetric method for determination of serum triglycerides with lipoprotein lipase and glycerol dehydrogenase

Clinica Chimica Acta; International Journal of Clinical Chemistry
M SugiuraT Kuratsu

Abstract

A simplified enzymic procedure to determine accurately serum triglycerides is described. Serum triglycerides are hydrolyzed completely to free fatty acids and glycerol by lipoprotein lipase from Pseudomonas fluorescens. The released glycerol is oxidized with glycerol dehydrogenase from Erwinia aroideae in the presence of NAD+, were the reduction of the enzyme-linked NAD+ is coupled to the reduction of nitro blue tetrazolium as a chromogenic indicator with phenazine methosulfate serving as an intermediate electron carrier of NADH. The absorbance at 570 nm is measured. The method requies only 20 microliter of serum and a 10-min incubation and is rapid and simple. The present method offers the measurement of a high concentration of triglyceride up to 1000 mg/dl serum. The results obtained by the present method show good correlation with those obtained by the glycerol kinase method (correlation coefficient, 0.989) or the acetylacetone method (correlation coefficient, 0.979). These results suggest that the proposed method will be utilized as a method or routine clinical test.

Citations

Dec 31, 2011·Environmental Health and Preventive Medicine·Terumi KogawaIkuo Kashiwakura
Oct 15, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·K KajinamiR Takeda
May 22, 2007·Metabolism: Clinical and Experimental·Naoki HarikaiAtsushi Ichikawa
Apr 3, 2004·Acta Obstetricia Et Gynecologica Scandinavica·Andrea Luigi TranquilliLaura Mazzanti
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Related Concepts

Colorimetry
Drug Stability
Formazans
Glycerin
Lipoprotein LIPASE
Study, Methodological
Sugar Alcohol Dehydrogenases
Tensides
Triglycerides

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