A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice

Abstract

References

Managing meiotic recombination in plant breeding

A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.

Multiplex genome engineering using CRISPR/Cas systems

One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

Targeted genome modification of crop plants using a CRISPR-Cas system

CRISPR-Cas systems for editing, regulating and targeting genomes

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

Citations

CRISPR/Cas9 for plant genome editing: accomplishments, problems and prospects

Expanding the Range of CRISPR/Cas9 Genome Editing in Rice

Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice

Heritability of targeted gene modifications induced by plant-optimized CRISPR systems

Targeted modification of plant genomes for precision crop breeding

Novel rice mutants overexpressing the brassinosteroid catabolic gene CYP734A4

Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922

Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice

CRISPR/Cas9: An RNA-guided highly precise synthetic tool for plant genome editing

TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis

OsSPL regulates meiotic fate acquisition in rice

A mitogen-activated protein kinase phosphatase influences grain size and weight in rice

The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice

A strategy for generating rice apomixis by gene editing

Pooled CRISPR/Cas9 reveals redundant roles of plastidial phosphoglycerate kinases in carbon fixation and metabolism

A critical look on CRISPR-based genome editing in plants

A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice

Characterization of the CRM Gene Family and Elucidating the Function of OsCFM2 in Rice.

The heterochronic gene Oryza sativa LIKE HETEROCHROMATIN PROTEIN 1 modulates miR156b/c/i/e levels.

OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice

Rapid Evolution of Manifold CRISPR Systems for Plant Genome Editing

Increasing the efficiency of CRISPR-Cas9-VQR precise genome editing in rice

iTRAQ-Based Analysis of Proteins Co-Regulated by Brassinosteroids and Gibberellins in Rice Embryos during Seed Germination

Evaluation of differential qPE9-1/DEP1 protein domains in rice grain length and weight variation

Defective Microspore Development 1 is required for microspore cell integrity and pollen wall formation in rice.

GS9 acts as a transcriptional activator to regulate rice grain shape and appearance quality

Rapid and user-friendly open-source CRISPR/Cas9 system for single- or multi-site editing of tomato genome

OsCDC48/48E complex is required for plant survival in rice (Oryza sativa L.)

ScCas9 recognizes NNG protospacer adjacent motif in genome editing of rice

QTL editing confers opposing yield performance in different rice varieties

Plant genome editing with TALEN and CRISPR

A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in Arabidopsis

xCas9 expands the scope of genome editing with reduced efficiency in rice

Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes.

Multiplex QTL editing of grain-related genes improves yield in elite rice varieties

Mutagenesis in Rice: The Basis for Breeding a New Super Plant

CRISPR Tools for Physiology and Cell State Changes: Potential of Transcriptional Engineering and Epigenome Editing.

Expanding the scope of genome editing with SpG and SpRY variants in rice.

CRISPR-based tools for plant genome engineering.

Cytokinin oxidase/dehydrogenase OsCKX11 coordinates source and sink relationship in rice by simultaneous regulation of leaf senescence and grain number.

DEEP GREEN PANICLE1 suppresses GOLDEN2-LIKE activity to reduce chlorophyll synthesis in rice glumes.

Improving rice eating and cooking quality by coordinated expression of the major starch synthesis-related genes, SSII and Wx, in endosperm.

An Improved CRISPR/Cas9 System for Genome Editing in Populus by Using Mannopine Synthase (MAS) Promoter.

Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions.

Rice G protein γ subunit qPE9-1 modulates root elongation for phosphorus uptake by involving 14-3-3 protein OsGF14b and plasma membrane H+ -ATPase.

OsCUL3a-associated molecular switches have functions in cell metabolism, cell death, and disease resistance

RGN1 controls grain number and shapes panicle architecture in rice.

Concurrent Disruption of Genetic Interference and Increase of Genetic Recombination Frequency in Hybrid Rice Using CRISPR/Cas9.

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