A simple, cross-linked collagen tissue substitute for corneal implantation

Investigative Ophthalmology & Visual Science
Yuwen LiuMay Griffith

Abstract

To develop a simple corneal substitute from cross-linked collagen. Porcine type I collagen (10%; pH 5), was mixed with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The final homogenous solution was molded to corneal dimensions, cured, and then implanted into rabbits and minipigs by lamellar keratoplasty. The implants were followed for up to 6 months after surgery. Clinical examinations of the cornea included detailed slit lamp biomicroscopy, in vivo confocal microscopy, topography and esthesiometry for nerve function. Histopathologic examinations were also performed on rabbit corneas harvested after 6 months. Cross-linked collagen (refractive index, 1.35) had optical clarity superior to human corneas. Implanted into rabbit and porcine corneas, only 1 of 24 of the surgical corneas showed a slight haze at 6 months after surgery. All other implants showed no adverse reactions and remained optically clear. Topography showed a smooth surface and a profile similar to that of the contralateral nonsurgical eye. The implanted matrices promoted regeneration of corneal cells, tear film, and nerves. Touch sensitivity was restored, indicating some restoration of function. The corneas with implants show...Continue Reading

Citations

Jun 12, 2008·Journal of Materials Science. Materials in Medicine·Wenguang LiuFengfu Li
Jun 10, 2010·In Vitro Cellular & Developmental Biology. Animal·Yi ShaoZuguo Liu
Mar 13, 2008·Tissue Engineering. Part a·Rachael A B Crabb, Allison Hubel
Jul 1, 2010·Tissue Engineering. Part a·Chao DengErik J Suuronen
May 4, 2011·Tissue Engineering. Part a·Ryou WatanabeKohji Nishida
Dec 18, 2012·Tissue Engineering. Part a·Shane BrowneAbhay Pandit
Mar 3, 2009·Tissue Engineering. Part C, Methods·Joo Youn OhJin Hak Lee
Jun 4, 2008·Cornea·Christopher R McLaughlinMay Griffith
Jul 3, 2010·Journal of Biomaterials Science. Polymer Edition·Yuji TanakaKohji Nishida
Aug 30, 2008·Journal of Biomaterials Science. Polymer Edition·M A Princz, H Sheardown
Apr 23, 2008·Pediatric Research·Ajay ShahElizabeth Orwin
Nov 13, 2014·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·T FuchslugerB Bachmann
Aug 11, 2015·Journal of Functional Biomaterials·Martina MiottoChe J Connon
Jun 12, 2009·Cutaneous and Ocular Toxicology·Chris J SompsGareth Phillips
Aug 28, 2012·Biomaterials·Xiomara Calderón-ColónMorgana M Trexler
Nov 9, 2010·Biomaterials·Yuji TanakaKohji Nishida
Oct 17, 2009·Current Opinion in Biotechnology·Hannah Levis, Julie T Daniels
Dec 14, 2007·Biomaterials·Wenguang LiuFengfu Li
Jan 15, 2008·Experimental Eye Research·Federico Castro-Muñozledo
Apr 24, 2007·Journal of Shoulder and Elbow Surgery·Augustus D MazzoccaRobert A Arciero
Mar 14, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Winnette McIntosh AmbroseJennifer Elisseeff
Mar 22, 2008·Journal of Biomedical Materials Research. Part a·Mehrdad RafatMay Griffith
May 31, 2008·Journal of Tissue Engineering and Regenerative Medicine·Subhadra DravidaGeeta K Vemuganti
May 7, 2010·Clinical and Translational Science·Per FagerholmMay Griffith
Dec 6, 2011·Acta Ophthalmologica·Efdal YoeruekPeter Szurman
Apr 20, 2010·Clinical & Experimental Ophthalmology·Ahmed GomaaChristopher Liu
Sep 22, 2015·ACS Applied Materials & Interfaces·Andri K RiauJodhbir S Mehta

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