PMID: 3214677Sep 1, 1988Paper

A simple desalting procedure for fast atom bombardment mass spectrometry

Biomedical & Environmental Mass Spectrometry
D C Moon, J A Kelley

Abstract

A simple and efficient procedure for desalting samples prior to fast atom bombardment mass spectrometry utilizes low-pressure reverse-phase chromatography with disposable high-capacity mini-columns. Microscale (0.1-10 mumol) sample purification with high analyte recovery is possible for nucleosides, nucleotides and peptides. Salt removal has been evaluated both by conductivity and mass spectral methods and is greater than 99% for the model compounds cytidine, adenosine and AMP, even when they are contaminated with a 10 molar excess of NaCl. This procedure is also applicable to a range of small peptides as demonstrated with pyro-Glu-His-Gly and methionine enkephalin.

Citations

Jul 1, 1994·Journal of the American Society for Mass Spectrometry·M R Emmett, R M Caprioli
Jan 19, 2013·Journal of Chromatographic Science·Qian LiuJeffrey N Agar
Feb 3, 2016·Methods : a Companion to Methods in Enzymology·Sarah TrimpinPaul M Stemmer
Jun 1, 1990·Analytical Chemistry·R E SynovecC N Renn
Jul 1, 1990·Journal of Bacteriology·D McLagganW Epstein

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