PMID: 7581325Sep 1, 1995Paper

A simple, efficient technique for freezing Tetrahymena thermophila

The Journal of Eukaryotic Microbiology
D Cassidy-HanleyP J Bruns

Abstract

We have developed a simple, efficient procedure for the long term freezing of Tetrahymena thermophila in liquid nitrogen. This technique yields excellent recovery of viable cells with all strains tested and does not require the use of a controlled rate low temperature freezer. To optimize the freezing technique, we have examined the effects of varying a number of parameters, including the physiological state of the cells prior to freezing, the time of exposure to cryoprotectant, and the rate of freezing and thawing. The frequency of viable cell recovery following freezing using this technique has been tested for a variety of different cell lines.

References

Jun 1, 1973·Cryobiology·J Farrant, G J Morris
Mar 1, 1957·Genetics·D L Nanney

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Citations

Dec 7, 2010·The Journal of Eukaryotic Microbiology·Kotaro MoriTetsuya Yomo
Jun 5, 2002·The Journal of Eukaryotic Microbiology·Jeffrey S FillinghamRonald E Pearlman
Jan 31, 2006·The Journal of Eukaryotic Microbiology·Alexander J BurdettePhillip E Ryals
Aug 20, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Maria L Sanchez GranelAlejandro D Nusblat

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