A simple enzyme-linked immunoassay of human carbonic anhydrase I for the study of developing erythroid cells

Clinica Chimica Acta; International Journal of Clinical Chemistry
T KondoY Kawakami

Abstract

A simple and rapid enzyme-linked immunoassay for human erythrocyte carbonic anhydrase isozyme I was developed. The assay was found to be sensitive enough for the detection of nanogram amounts of the enzyme in incubation mixtures. The first incubation with anti-human carbonic anhydrase I IgG was carried out for 6 hours at room temperature. The second incubation of the enzyme was carried out in the presence of goat plasma C1q coupled with peroxidase for 1 h at room temperature. The enzymatic reaction was performed for 30 min using 2,2'-azino-di(3-ethyl-benzthiazoline sulfonate) as a substrate, and absorbance at 414 nm was recorded. Carbonic anhydrase I was assayed on the range of 1 to 200 ng/ml using this method. The levels of carbonic anhydrase I in K562 cells induced by erythropoietin and in other blood cells were determined. This enzyme-linked immunoassay has application for the study of developing erythroid cells.

References

Sep 1, 1978·The Journal of Clinical Investigation·T KondoM Murao
May 1, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·T KondoE Takakuwa
May 7, 1976·Analytical Biochemistry·N TaniguchiE Takakuwa
Jul 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·T KondoY Kawakami
Mar 19, 1970·Biochimica Et Biophysica Acta·J A Edwards
Jan 1, 1984·Annals of the New York Academy of Sciences·S C WeilR K Hirata

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Citations

Apr 1, 1996·Clinical Biochemistry·S Aliakbar, P R Brown
Oct 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J FerrièresS Lussier-Cacan
Oct 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·L C LyuE J Schaefer

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