PMID: 64003Nov 1, 1976Paper

A simple fluorescence staining technique for the differentiation of human tissue transplanted into nude mice

Stain Technology
H J Müller

Abstract

Human and mouse nuclei can be distinguished by differences in the constitutive heterochromatin when stained with quinacrine dihydrochloride. With the staining method described, mouse heterochromatin during interphase appears as brilliant fluorescent chromocenters. By replacing the commonly used aqueous buffer mounting medium with a xylene-diluted synthetic resin, the haziness of the nuclear fluorescence is eliminated thus allowing identification of the heterochromatin pattern in histological preparations. A requirement for the definite identification of cells of human or murine origin in the nude mouse is the knowledge that the heterochromatin arrangements changes according to the stage of differentiation of the cell of the position of a particular nucleus within the cell cycle.

References

Mar 1, 1975·Experimental Cell Research·G C MoserE Robbins
Sep 1, 1975·The Journal of Cell Biology·F G MoserF H Ruddle
Jan 1, 1971·Chromosoma·T C HsuB R Brinkley
Dec 23, 1972·Lancet·H P Klinger, G C Moser
Jan 1, 1974·Humangenetik·F Vogel, T M Schroeder
Sep 1, 1957·Stain Technology·H P KLINGER, K S LUDWIG

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Citations

Jan 1, 1982·Anatomy and Embryology·P Groscurth, G Töndury
May 1, 2007·Journal of Cataract and Refractive Surgery·Liliana WernerNick Mamalis
Oct 31, 1998·Journal of Cataract and Refractive Surgery·L P WernerG Renard
Nov 14, 1997·Journal of Cataract and Refractive Surgery·L P WernerG Renard
Mar 24, 2012·Journal of Cataract and Refractive Surgery·Kathleen L KrenzerMary E Richardson
Dec 1, 1983·The British Journal of Ophthalmology·M J MannisD R May
Jun 30, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ajit ThakurGonzalo Hortelano
Jul 1, 1988·The British Journal of Ophthalmology·D S RootmanP K Basu
May 1, 1995·The British Journal of Ophthalmology·G A Peyman

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