A simple high-performance liquid chromatography method for the determination of throat-burning oleocanthal with probated antiinflammatory activity in extra virgin olive oils

Journal of Agricultural and Food Chemistry
John Impellizzeri, Jianming Lin

Abstract

A high-performance liquid chromatography (HPLC) method was developed to quantitatively analyze oleocanthal in extra virgin olive oils. Oleocanthal, a deacetoxy ligstroside aglycone, is known to be responsible for the back of the throat irritation of olive oils and to have probated antiinflamatory activity. Oleocanthal was isolated from small amounts of olive oil sample (1 g) by liquid-liquid extraction. Hexane-acetonitrile was found to be the best solvent system to extract oleocanthal from the oil matrix. The solvent extract was analyzed by reversed-phase HPLC with UV detection at 278 nm. Chromatogaphic separation of oleocanthal from other extracted compounds and of the two geometric isomers of oleocanthal was achieved by an elution gradient with acetonitrile and water. Both the external standard calibration curve and the internal standard calibration curve were established, and quantitation using both calibration curves gave essentially the same result. The reproducibility (RSD = 4.7%), recovery (> 95%), and limit of quantitation (< 1 microg/g) were also determined. Concentrations of oleacanthal in 10 selected throat-burning extra virgin olive oils were determined using the method (ranged from 22 to 190 microg/g) with external...Continue Reading

Citations

Sep 24, 2011·Journal of Agricultural and Food Chemistry·Jesus Lozano-SánchezAlberto Fernández-Gutiérrez
Dec 19, 2008·Critical Reviews in Food Science and Nutrition·Sara CiceraleRussell S J Keast
Mar 11, 2011·Natural Product Research·S CiceraleR S J Keast
Jul 20, 2010·Annual Review of Analytical Chemistry·Peter Schoenmakers
Oct 13, 2014·Food Chemistry·Hamid-Reza AdhamiLiselotte Krenn
Sep 26, 2015·Sensors·Marco SantonicoGiorgio Pennazza
Sep 17, 2014·International Journal of Molecular Sciences·Jesús Lozano-SánchezElena Ibáñez
Feb 22, 2011·Neurochemistry International·Anthony DaccachePhilippe Cotelle
Jun 25, 2009·Journal of Neurochemistry·Wenkai LiVirginia M-Y Lee
Dec 21, 2011·Molecular Nutrition & Food Research·Aida SerraMaria-José Motilva
Feb 27, 2014·Journal of Natural Products·Konstantina VougogiannopoulouBrigitte Deguin
Mar 22, 2019·Natural Product Research·Vita Di Stefano, Maria Grazia Melilli
Sep 27, 2018·International Journal of Molecular Sciences·Antonio Segura-Carretero, Jose Antonio Curiel
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ilario LositoTommaso R I Cataldi
Jan 31, 2021·International Journal of Molecular Sciences·Maria Rita EmmaAntonella Cusimano
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Assunta EspositoMonica Scognamiglio
May 26, 2018·Journal of Agricultural and Food Chemistry·Rita CelanoMariateresa Russo

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